1ATO

THE STRUCTURE OF THE ISOLATED, CENTRAL HAIRPIN OF THE HDV ANTIGENOMIC RIBOZYME, NMR, 10 STRUCTURES

1ATO の概要

• エントリーDOI: 10.2210/pdb1ato/pdb
• 分子名称: RNA (5'-R(*GP*GP*CP*AP*CP*CP*UP*CP*CP*UP*CP*GP*CP*GP*GP*UP*GP*CP*C)-3') (1 entity in total)
• 機能のキーワード: central loop, hepatitis delta virus, ribozyme, rna hairpin, rna
• 由来する生物種: Hepatitis delta virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6020.62

構造登録者

Kolk, M.H.,Heus, H.A.,Hilbers, C.W. (登録日: 1997-08-14, 公開日: 1997-11-12, 最終更新日: 2024-05-22)

主引用文献

Kolk, M.H.,Heus, H.A.,Hilbers, C.W.
The structure of the isolated, central hairpin of the HDV antigenomic ribozyme: novel structural features and similarity of the loop in the ribozyme and free in solution.
EMBO J., 16:3685-3692, 1997

PubMed Abstract: The structure of an RNA hairpin containing a seven-nucleotide loop that is present in the self-cleaving sequence of hepatitis delta virus antigenomic RNA was determined by high resolution NMR spectroscopy. The loop, which is composed of only one purine and six pyrimidines, has a suprisingly stable structure, mainly supported by sugar hydroxyl hydrogen bonds and base-base and base-phosphate stacking interactions. Compared with the structurally well-determined, seven-membered anticodon loop in tRNA, the sharp turn which affects the required 180 degrees change in direction of the sugar-phosphate backbone in the loop is shifted one nucleotide in the 3' direction. This change in direction can be characterized as a reversed U-turn. It is expected that the reversed U-turn may be found frequently in other molecules as well. There is evidence for a new non-Watson-Crick UC base pair formed between the first and the last residue in the loop, while most of the other bases in the loop are pointing outwards making them accessible to solvent. From chemical modification, mutational and photocrosslinking studies, a similar picture develops for the structure of the hairpin in the active ribozyme indicating that the loop structure in the isolated hairpin and in the ribozyme is very similar.

PubMed: 9218809
DOI: 10.1093/emboj/16.12.3685

実験手法

SOLUTION NMR