1AQQ

AG-SUBSTITUTED METALLOTHIONEIN FROM SACCHAROMYCES CEREVISIAE, NMR, 10 STRUCTURES

1AQQ の概要

• エントリーDOI: 10.2210/pdb1aqq/pdb
• 分子名称: AG-METALLOTHIONEIN, SILVER ION (2 entities in total)
• 機能のキーワード: metallothionein, copper detoxification, metal-thiolate cluster
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5030.73

構造登録者

Peterson, C.W.,Narula, S.S.,Armitage, I.M. (登録日: 1997-07-31, 公開日: 1997-12-24, 最終更新日: 2024-05-22)

主引用文献

Peterson, C.W.,Narula, S.S.,Armitage, I.M.
3D solution structure of copper and silver-substituted yeast metallothioneins.
FEBS Lett., 379:85-93, 1996

PubMed Abstract: 3D solution structural calculations for yeast silver(I)-substituted metallothionein (MT) and native copper(I) MT were completed using experimentally determined NOE and dihedral angle constraints, in conjunction with experimentally derived metal-to-Cys connectivities for AgMT which were assumed identical for CuMT. For the first 40 residues in both structures, the polypeptide backbone wraps around the metal cluster in two large parallel loops separated by a deep cleft containing the metal cluster. Minor differences between the two structures include differences in hydrogen bonds and the orientation of the N-terminus with the overall protein volume conserved to within 6.5%.

PubMed: 8566237
DOI: 10.1016/0014-5793(95)01492-6

実験手法

SOLUTION NMR