1AP8

TRANSLATION INITIATION FACTOR EIF4E IN COMPLEX WITH M7GDP, NMR, 20 STRUCTURES

1AP8 の概要

• エントリーDOI: 10.2210/pdb1ap8/pdb
• 分子名称: TRANSLATION INITIATION FACTOR EIF4E, 7N-METHYL-8-HYDROGUANOSINE-5'-DIPHOSPHATE (2 entities in total)
• 機能のキーワード: rna cap, translation initiation factor
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24745.44

構造登録者

Matsuo, H.,Li, H.,Mcguire, A.M.,Fletcher, M.,Gingras, A.C.,Sonenberg, N.,Wagner, G. (登録日: 1997-07-25, 公開日: 1998-01-28, 最終更新日: 2024-03-06)

主引用文献

Matsuo, H.,Li, H.,McGuire, A.M.,Fletcher, C.M.,Gingras, A.C.,Sonenberg, N.,Wagner, G.
Structure of translation factor eIF4E bound to m7GDP and interaction with 4E-binding protein.
Nat.Struct.Biol., 4:717-724, 1997

PubMed Abstract: eIF4E, the mRNA cap binding protein, is a master switch that controls eukaryotic translation. To be active, it must bind eIF4G and form the eIF4F complex, which also contains eIF4A. Translation is downregulated by association of eIF4E with 4E-BP, which occupies the eIF4G binding site. Signalling events acting on 4E-BP cause it to dissociate from eIF4E, and eIF4E is then free to bind eIF4G to form the active eIF4F complex. We have solved the structure of the yeast eIF4E/m7Gpp complex in a CHAPS micelle. We determined the position of the second nucleotide in a complex with m7GpppA, and identified the 4E-BP binding site. eIF4E has a curved eight-stranded antiparallel beta-sheet, decorated with three helices on the convex face and three smaller helices inserted in connecting loops. The m7G of the cap is intercalated into a stack of tryptophans in the concave face. The 4E-BP binding site is located in a region encompassing one edge of the beta-sheet, the adjacent helix a2 and several regions of non-regular secondary structure. It is adjacent to, but does not overlap the cap-binding site.

PubMed: 9302999
DOI: 10.1038/nsb0997-717

実験手法

SOLUTION NMR