1AOO

AG-SUBSTITUTED METALLOTHIONEIN FROM SACCHAROMYCES CEREVISIAE, NMR, MINIMIZED AVERAGE STRUCTURE

Summary for 1AOO

• Entry DOI: 10.2210/pdb1aoo/pdb
• Descriptor: AG-METALLOTHIONEIN, SILVER ION (2 entities in total)
• Functional Keywords: metallothionein, copper detoxification, metal-thiolate cluster
• Biological source: Saccharomyces cerevisiae (baker's yeast)
• Total number of polymer chains: 1
• Total formula weight: 5030.73

Authors

Peterson, C.W.,Narula, S.S.,Armitage, I.M. (deposition date: 1997-07-08, release date: 1997-12-24, Last modification date: 2024-05-22)

Primary citation

Peterson, C.W.,Narula, S.S.,Armitage, I.M.
3D solution structure of copper and silver-substituted yeast metallothioneins.
FEBS Lett., 379:85-93, 1996

PubMed Abstract: 3D solution structural calculations for yeast silver(I)-substituted metallothionein (MT) and native copper(I) MT were completed using experimentally determined NOE and dihedral angle constraints, in conjunction with experimentally derived metal-to-Cys connectivities for AgMT which were assumed identical for CuMT. For the first 40 residues in both structures, the polypeptide backbone wraps around the metal cluster in two large parallel loops separated by a deep cleft containing the metal cluster. Minor differences between the two structures include differences in hydrogen bonds and the orientation of the N-terminus with the overall protein volume conserved to within 6.5%.

PubMed: 8566237
DOI: 10.1016/0014-5793(95)01492-6

Experimental method

SOLUTION NMR