1AMO

THREE-DIMENSIONAL STRUCTURE OF NADPH-CYTOCHROME P450 REDUCTASE: PROTOTYPE FOR FMN-AND FAD-CONTAINING ENZYMES

1AMO の概要

• エントリーDOI: 10.2210/pdb1amo/pdb
• 分子名称: NADPH-CYTOCHROME P450 REDUCTASE, FLAVIN-ADENINE DINUCLEOTIDE, FLAVIN MONONUCLEOTIDE, ... (5 entities in total)
• 機能のキーワード: flavoprotein, nitric oxide synthase, oxidoreductase
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Endoplasmic reticulum membrane; Peripheral membrane protein: P00388
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 143913.88

構造登録者

Wang, M.,Roberts, D.L.,Paschke, R.,Shea, T.M.,Masters, B.S.S.,Kim, J.J.P. (登録日: 1997-06-17, 公開日: 1998-06-17, 最終更新日: 2024-02-07)

主引用文献

Wang, M.,Roberts, D.L.,Paschke, R.,Shea, T.M.,Masters, B.S.,Kim, J.J.
Three-dimensional structure of NADPH-cytochrome P450 reductase: prototype for FMN- and FAD-containing enzymes.
Proc.Natl.Acad.Sci.USA, 94:8411-8416, 1997

PubMed Abstract: Microsomal NADPH-cytochrome P450 reductase (CPR) is one of only two mammalian enzymes known to contain both FAD and FMN, the other being nitric-oxide synthase. CPR is a membrane-bound protein and catalyzes electron transfer from NADPH to all known microsomal cytochromes P450. The structure of rat liver CPR, expressed in Escherichia coli and solubilized by limited trypsinolysis, has been determined by x-ray crystallography at 2.6 A resolution. The molecule is composed of four structural domains: (from the N- to C- termini) the FMN-binding domain, the connecting domain, and the FAD- and NADPH-binding domains. The FMN-binding domain is similar to the structure of flavodoxin, whereas the two C-terminal dinucleotide-binding domains are similar to those of ferredoxin-NADP+ reductase (FNR). The connecting domain, situated between the FMN-binding and FNR-like domains, is responsible for the relative orientation of the other domains, ensuring the proper alignment of the two flavins necessary for efficient electron transfer. The two flavin isoalloxazine rings are juxtaposed, with the closest distance between them being about 4 A. The bowl-shaped surface near the FMN-binding site is likely the docking site of cytochrome c and the physiological redox partners, including cytochromes P450 and b5 and heme oxygenase.

PubMed: 9237990
DOI: 10.1073/pnas.94.16.8411

実験手法

X-RAY DIFFRACTION (2.6 Å)