1ALX

GRAMICIDIN D FROM BACILLUS BREVIS (METHANOL SOLVATE)

1ALX の概要

• エントリーDOI: 10.2210/pdb1alx/pdb
• 関連するPDBエントリー: 1AL4 1ALZ 1AV2 1BDW 1C4D 1GMK 1GRM 1JNO 1JO3 1JO4 1KQE 1MAG 1MIC 1NG8 1NRM 1NRU 1NT5 1NT6 1TK2 1TKQ 1W5U 2IZQ 2XDC 3L8L
• 関連するBIRD辞書のPRD_ID: PRD_000152
• 分子名称: GRAMICIDIN A, METHANOL (3 entities in total)
• 機能のキーワード: antibiotic, gramicidin, antifungal, antibacterial, abtibiotic, membrane ion channel, linear gramicidin
• 由来する生物種: BREVIBACILLUS BREVIS; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 4382.39

構造登録者

Burkhart, B.M.,Langs, D.A.,Smith, G.D.,Courseille, C.,Precigoux, G.,Hospital, M.,Pangborn, W.A.,Duax, W.L. (登録日: 1997-06-05, 公開日: 1998-03-04, 最終更新日: 2024-10-30)

主引用文献

Burkhart, B.M.,Gassman, R.M.,Langs, D.A.,Pangborn, W.A.,Duax, W.L.
Heterodimer Formation and Crystal Nucleation of Gramicidin D
Biophys.J., 75:2135-, 1998

PubMed Abstract: The linear pentadecapeptide antibiotic gramicidin D is a heterogeneous mixture of six components. Precise refinements of three-dimensional structures of naturally occurring gramicidin D in crystals obtained from methanol, ethanol, and n-propanol demonstrate the unexpected presence of stable left-handed antiparallel double-helical heterodimers that vary with the crystallization solvent. The side chains of Trp residues in the three structures exhibit sequence-specific patterns of conformational preference. Tyr substitution for Trp at position 11 appears to favor beta ribbon formation and stabilization of the antiparallel double helix that acts as a template for gramicidin folding and nucleation of different crystal forms. The fact that a minor component in a heterogeneous mixture influences aggregation and crystal nucleation has potential applications to other systems in which anomalous behavior is exhibited by aggregation of apparently homogeneous materials, such as the enigmatic behavior of prion proteins.

PubMed: 9788907
DOI: 10.1016/S0006-3495(98)77656-8

実験手法

X-RAY DIFFRACTION (1.2 Å)