1ALV

CALCIUM BOUND DOMAIN VI OF PORCINE CALPAIN

1ALV の概要

• エントリーDOI: 10.2210/pdb1alv/pdb
• 分子名称: CALPAIN, CALCIUM ION (3 entities in total)
• 機能のキーワード: calcium binding, calmodulin like, domain of cystein protease
• 由来する生物種: Sus scrofa (pig)
• 細胞内の位置: Cytoplasm (By similarity): P04574
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 40087.58

構造登録者

Narayana, S.V.L.,Lin, G.,Chattopadhyay, D.,Maki, M. (登録日: 1997-06-03, 公開日: 1998-06-03, 最終更新日: 2024-02-07)

主引用文献

Lin, G.D.,Chattopadhyay, D.,Maki, M.,Wang, K.K.,Carson, M.,Jin, L.,Yuen, P.W.,Takano, E.,Hatanaka, M.,DeLucas, L.J.,Narayana, S.V.
Crystal structure of calcium bound domain VI of calpain at 1.9 A resolution and its role in enzyme assembly, regulation, and inhibitor binding.
Nat.Struct.Biol., 4:539-547, 1997

PubMed Abstract: The three dimensional structure of calcium-bound domain VI of porcine calpain has been determined to 1.9 A resolution. The crystal structure reveals five EF-hands, one more than previously suggested. There are two EF-hand pairs, one pair (EF1-EF2) displays an 'open' conformation and the other (EF3-EF4) a 'closed' conformation. Unusually, a calcium atom is found at the C-terminal end of the calcium binding loop of EF4. With two additional residues in the calcium binding loop, the fifth EF-hand (EF5) is in a 'closed' conformation. EF5 pairs up with the corresponding fifth EF-hand of a non-crystallographically related molecule. Considering the EF5's role in a homodimer formation of domain VI, we suggest a model for the assembly of heterodimeric calpain. The crystal structure of a Ca2+ bound domain VI-inhibitor (PD150606) complex has been refined to 2.1 A resolution. A possible mode for calpain inhibition is discussed.

PubMed: 9228946
DOI: 10.1038/nsb0797-539

実験手法

X-RAY DIFFRACTION (1.9 Å)