1AL7

THREE-DIMENSIONAL STRUCTURES OF GLYCOLATE OXIDASE WITH BOUND ACTIVE-SITE INHIBITORS

1AL7 の概要

• エントリーDOI: 10.2210/pdb1al7/pdb
• 分子名称: GLYCOLATE OXIDASE, FLAVIN MONONUCLEOTIDE, 4-CARBOXY-5-(1-PENTYL)HEXYLSULFANYL-1,2,3-TRIAZOLE, ... (4 entities in total)
• 機能のキーワード: flavoprotein, drug design, inhibitor binding
• 由来する生物種: Spinacia oleracea (spinach)
• 細胞内の位置: Peroxisome: P05414
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40092.13

構造登録者

Stenberg, K.,Lindqvist, Y. (登録日: 1997-06-12, 公開日: 1997-11-12, 最終更新日: 2024-02-07)

主引用文献

Stenberg, K.,Lindqvist, Y.
Three-dimensional structures of glycolate oxidase with bound active-site inhibitors.
Protein Sci., 6:1009-1015, 1997

PubMed Abstract: A key step in plant photorespiration, the oxidation of glycolate to glyoxylate, is carried out by the peroxisomal flavoprotein glycolate oxidase (EC 1.1.3.15). The three-dimensional structure of this alpha/beta barrel protein has been refined to 2 A resolution (Lindqvist Y. 1989. J Mol Biol 209:151-166). FMN dependent glycolate oxidase is a member of the family of alpha-hydroxy acid oxidases. Here we describe the crystallization and structure determination of two inhibitor complexes of the enzyme, TKP (3-Decyl-2,5-dioxo-4-hydroxy-3-pyrroline) and TACA (4-Carboxy-5-(1-pentyl)hexylsulfanyl-1,2,3-triazole). The structure of the TACA complex has been refined to 2.6 A resolution and the TKP complex, solved with molecular replacement, to 2.2 A resolution. The Rfree for the TACA and TKP complexes are 24.2 and 25.1%, respectively. The overall structures are very similar to the unliganded holoenzyme, but a closer examination of the active site reveals differences in the positioning of the flavin isoalloxazine ring and a displaced flexible loop in the TKP complex. The two inhibitors differ in binding mode and hydrophobic interactions, and these differences are reflected by the very different Ki values for the inhibitors, 16 nM for TACA and 4.8 microM for TKP. Implications of the structures of these enzyme-inhibitor complexes for the model for substrate binding and catalysis proposed from the holo-enzyme structure are discussed.

PubMed: 9144771

実験手法

X-RAY DIFFRACTION (2.6 Å)