1AL3

COFACTOR BINDING FRAGMENT OF CYSB FROM KLEBSIELLA AEROGENES

1AL3 の概要

• エントリーDOI: 10.2210/pdb1al3/pdb
• 分子名称: CYS REGULON TRANSCRIPTIONAL ACTIVATOR CYSB, SULFATE ION (3 entities in total)
• 機能のキーワード: lysr family, cysteine biosynthesis, transcription regulation
• 由来する生物種: Klebsiella aerogenes
• 細胞内の位置: Cytoplasm: P45600
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36104.17

構造登録者

Verschueren, K.H.G.,Tyrrell, R.,Wilkinson, A.J. (登録日: 1997-06-10, 公開日: 1997-12-24, 最終更新日: 2024-02-07)

主引用文献

Tyrrell, R.,Verschueren, K.H.,Dodson, E.J.,Murshudov, G.N.,Addy, C.,Wilkinson, A.J.
The structure of the cofactor-binding fragment of the LysR family member, CysB: a familiar fold with a surprising subunit arrangement.
Structure, 5:1017-1032, 1997

PubMed Abstract: CysB is a tetrameric protein of identical subunits (M(r) = 36,000) which controls the expression of genes associated with the biosynthesis of cysteine in bacteria. CysB is both an activator and a repressor of transcription whose activity is responsive to the inducer N-acetylserine; thiosulphate and sulphide act as anti-inducers. CysB is a member of the LysR family of prokaryotic transcriptional regulatory proteins which share sequence similarities over approximately 280 residues including a putative helix-turn-helix DNA-binding motif at their N terminus. The aims of the present study were to explore further the complex molecular biology and curious ligand binding properties of CysB and to provide structural insights into the LysR family of proteins.

PubMed: 9309218
DOI: 10.1016/S0969-2126(97)00254-2

実験手法

X-RAY DIFFRACTION (1.8 Å)