1AK7

DESTRIN, NMR, 20 STRUCTURES

1AK7 の概要

• エントリーDOI: 10.2210/pdb1ak7/pdb
• 分子名称: DESTRIN (1 entity in total)
• 機能のキーワード: actin depolymerization factor
• 由来する生物種: Sus scrofa (pig)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19379.65

構造登録者

Hatanaka, H.,Moriyama, K.,Ogura, K.,Ichikawa, S.,Yahara, I.,Inagaki, F. (登録日: 1997-05-29, 公開日: 1997-10-15, 最終更新日: 2024-05-22)

主引用文献

Hatanaka, H.,Ogura, K.,Moriyama, K.,Ichikawa, S.,Yahara, I.,Inagaki, F.
Tertiary structure of destrin and structural similarity between two actin-regulating protein families.
Cell(Cambridge,Mass.), 85:1047-1055, 1996

PubMed Abstract: Destrin is an isoprotein of cofilin that regulates actin cytoskeleton in various eukaryotes. We determined the tertiary structure of destrin by triple-resonance multidimensional nuclear magnetic resonance. In spite of there being no significant amino acid sequence homology, we found that the folding of destrin was strikingly similar to that of repeated segments in the gelsolin family, which resulted in a new protein fold group. Sequential dissimilarity of the actin-binding helix of destrin to that of gelsolin explains the Ca2+-independent actin-binding of destrin. Possible mechanisms of phosphorylation-sensitive phosphoinositide-competitive actin binding, of pH-dependent filament severing, and of nuclear translocation with actin in response to stresses, are discussed on the basis of the tertiary structure.

PubMed: 8674111
DOI: 10.1016/S0092-8674(00)81305-7

実験手法

SOLUTION NMR