1AJP

PENICILLIN ACYLASE COMPLEXED WITH 2,5-DIHYDROXYPHENYLACETIC ACID

1AJP の概要

• エントリーDOI: 10.2210/pdb1ajp/pdb
• 分子名称: PENICILLIN AMIDOHYDROLASE, 2-(3,6-DIHYDROXYPHENYL)ACETIC ACID, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: antibiotic resistance, ligand induced conformational change, hydrolase
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Periplasm: P06875 P06875
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 86475.56

構造登録者

Done, S.H. (登録日: 1997-05-07, 公開日: 1997-11-12, 最終更新日: 2024-12-25)

主引用文献

Done, S.H.,Brannigan, J.A.,Moody, P.C.E.,Hubbard, R.E.
Ligand-induced conformational change in penicillin acylase.
J.Mol.Biol., 284:463-475, 1998

PubMed Abstract: The enzyme penicillin acylase (penicillin amidohydrolase EC 3.5.1. 11) catalyses the cleavage of the amide bond in the benzylpenicillin (penicillin G) side-chain to produce phenylacetic acid and 6-aminopenicillanic acid (6-APA). The enzyme is of great pharmaceutical importance, as the product 6-APA is the starting point for the synthesis of many semi-synthetic penicillin antibiotics. Studies have shown that the enzyme is specific for hydrolysis of phenylacetamide derivatives, but is more tolerant of features in the rest of the substrate. It is this property that has led to many other applications for the enzyme, and greater knowledge of the enzyme's structure and specificity could facilitate engineering of the enzyme, enhancing its potential for chemical and industrial applications. An extensive study of the binding of a series of phenylacetic acid derivatives has been carried out. A measure of the relative degree of inhibition of the enzyme by each of the compounds has been obtained using a competitive inhibition assay, and the structures of a number of these complexes have been determined by X-ray crystallography. The structures reveal a clear rationale for the observed kinetic results, but show also that some of the ligands cause a conformational change within the binding pocket. This change can generally be understood in terms of the size and orientation of the ligand within the active site.The results reveal that ligand binding in penicillin acylase is facilitated by certain amino acid residues that can adopt two distinct, energetically favourable positions in order to accommodate a variety of compounds within the active site. The structures of these complexes provide evidence for conformational changes in the substrate-binding region that may act as a switch in the mechanism of autocatalytic processing of this enzyme.

PubMed: 9813130
DOI: 10.1006/jmbi.1998.2180

実験手法

X-RAY DIFFRACTION (2.31 Å)