1AJF

SOLUTION STRUCTURE OF THE P5B STEM LOOP FROM A GROUP I INTRON COMPLEXED WITH COBALT (III) HEXAMMINE, NMR, MINIMIZED AVERAGE STRUCTURE

Summary for 1AJF

• Entry DOI: 10.2210/pdb1ajf/pdb
• Descriptor: RNA (5'-R(*GP*AP*CP*AP*GP*GP*GP*GP*AP*AP*AP*CP*UP*UP*UP*GP*UP*C)-3'), COBALT HEXAMMINE(III) (2 entities in total)
• Functional Keywords: ribonucleic acid, cobalt (iii) hexammine, metal binding, rna structure, rna
• Biological source: synthetic construct
• Total number of polymer chains: 1
• Total formula weight: 5973.63

Authors

Kieft, J.S.,Tinoco Junior, I. (deposition date: 1997-05-02, release date: 1997-07-07, Last modification date: 2024-05-22)

Primary citation

Kieft, J.S.,Tinoco Jr., I.
Solution structure of a metal-binding site in the major groove of RNA complexed with cobalt (III) hexammine.
Structure, 5:713-721, 1997

PubMed Abstract: Solvated metal ions are critical for the proper folding and function of RNA. Despite the importance of these ions, the details of specific metal ion-RNA interactions are poorly understood. The crystal structure of a group I intron ribozyme domain characterized several metal-binding sites in the RNA with osmium (III) hexammine bound in the major groove. A corresponding method for locating and characterizing metal-binding sites of RNA in solution is of obvious interest. NMR should be ideal for localizing metal hexammine ions bound to the RNA because of the large concentration of protons around the metal center.

PubMed: 9195889
DOI: 10.1016/S0969-2126(97)00225-6

Experimental method

SOLUTION NMR