1AJF

SOLUTION STRUCTURE OF THE P5B STEM LOOP FROM A GROUP I INTRON COMPLEXED WITH COBALT (III) HEXAMMINE, NMR, MINIMIZED AVERAGE STRUCTURE

1AJF の概要

• エントリーDOI: 10.2210/pdb1ajf/pdb
• 分子名称: RNA (5'-R(*GP*AP*CP*AP*GP*GP*GP*GP*AP*AP*AP*CP*UP*UP*UP*GP*UP*C)-3'), COBALT HEXAMMINE(III) (2 entities in total)
• 機能のキーワード: ribonucleic acid, cobalt (iii) hexammine, metal binding, rna structure, rna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5973.63

構造登録者

Kieft, J.S.,Tinoco Junior, I. (登録日: 1997-05-02, 公開日: 1997-07-07, 最終更新日: 2024-05-22)

主引用文献

Kieft, J.S.,Tinoco Jr., I.
Solution structure of a metal-binding site in the major groove of RNA complexed with cobalt (III) hexammine.
Structure, 5:713-721, 1997

PubMed Abstract: Solvated metal ions are critical for the proper folding and function of RNA. Despite the importance of these ions, the details of specific metal ion-RNA interactions are poorly understood. The crystal structure of a group I intron ribozyme domain characterized several metal-binding sites in the RNA with osmium (III) hexammine bound in the major groove. A corresponding method for locating and characterizing metal-binding sites of RNA in solution is of obvious interest. NMR should be ideal for localizing metal hexammine ions bound to the RNA because of the large concentration of protons around the metal center.

PubMed: 9195889
DOI: 10.1016/S0969-2126(97)00225-6

実験手法

SOLUTION NMR