1AII

ANNEXIN III

1AII の概要

• エントリーDOI: 10.2210/pdb1aii/pdb
• 分子名称: ANNEXIN III, CALCIUM ION, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: calcium/phospholipid binding protein, annexin, phospholipase a2 inhibitor, calcium-phospholipid binding protein complex
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36784.81

構造登録者

Lewit-Bentley, A.,Perron, B. (登録日: 1996-11-28, 公開日: 1997-03-12, 最終更新日: 2023-11-15)

主引用文献

Perron, B.,Lewit-Bentley, A.,Geny, B.,Russo-Marie, F.
Can enzymatic activity, or otherwise, be inferred from structural studies of annexin III?
J.Biol.Chem., 272:11321-11326, 1997

PubMed Abstract: Annexin III, a putative inositol (1,2)-phosphohydrolase, was co-crystallized with inositol 2-phosphate, the inhibitor of the reaction, and its structure was solved to 1.95 A resolution. No enzyme active site was observed in the structure. Assays for enzymatic activity were also negative. Search for annexin III-inositol phosphate interactions using the BIAcoreTM system revealed an affinity for inositol cyclic (1,2)-phosphate, suggesting annexin III may sequester the molecule in the cell. The BIAcoreTM system used with different phospholipids showed that annexin III displays specificity for phosphatidylethanolamine, but not for phosphatidylinositols. Interestingly, a molecule of ethanolamine was found bound to the protein in the crystal structure. Coupled with the fact that this is a particularly abundant phospholipid in granules specific to neutrophils, cells where annexin III is highly expressed, our finding could be pointing to a physiological role of annexin III.

PubMed: 9111038
DOI: 10.1074/jbc.272.17.11321

実験手法

X-RAY DIFFRACTION (1.95 Å)