1AIG

PHOTOSYNTHETIC REACTION CENTER FROM RHODOBACTER SPHAEROIDES IN THE D+QB-CHARGE SEPARATED STATE

1AIG の概要

• エントリーDOI: 10.2210/pdb1aig/pdb
• 分子名称: PHOTOSYNTHETIC REACTION CENTER (L SUBUNIT), PHOTOSYNTHETIC REACTION CENTER (M SUBUNIT), PHOTOSYNTHETIC REACTION CENTER (H SUBUNIT), ... (8 entities in total)
• 機能のキーワード: photosynthetic reaction center, integral membrane protein, charge separated
• 由来する生物種: Rhodobacter sphaeroides; 詳細
• 細胞内の位置: Cellular chromatophore membrane; Multi-pass membrane protein: P02954 P02953; Cellular chromatophore membrane; Single-pass membrane protein: P11846
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 202178.61

構造登録者

Stowell, M.H.B.,Mcphillips, T.M.,Soltis, S.M.,Rees, D.C.,Abresch, E.,Feher, G. (登録日: 1997-04-17, 公開日: 1997-10-22, 最終更新日: 2024-02-07)

主引用文献

Stowell, M.H.,McPhillips, T.M.,Rees, D.C.,Soltis, S.M.,Abresch, E.,Feher, G.
Light-induced structural changes in photosynthetic reaction center: implications for mechanism of electron-proton transfer.
Science, 276:812-816, 1997

PubMed Abstract: High resolution x-ray diffraction data from crystals of the Rhodobacter sphaeroides photosynthetic reaction center (RC) have been collected at cryogenic temperature in the dark and under illumination, and the structures were refined at 2.2 and 2.6 angstrom resolution, respectively. In the charge-separated D+QAQB- state (where D is the primary electron donor (a bacteriochlorophyll dimer), and QA and QB are the primary and secondary quinone acceptors, respectively), QB- is located approximately 5 angstroms from the QB position in the charge-neutral (DQAQB) state, and has undergone a 180 degrees propeller twist around the isoprene chain. A model based on the difference between the two structures is proposed to explain the observed kinetics of electron transfer from QA-QB to QAQB- and the relative binding affinities of the different ubiquinone species in the QB pocket. In addition, several water channels (putative proton pathways) leading from the QB pocket to the surface of the RC were delineated, one of which leads directly to the membrane surface.

PubMed: 9115209
DOI: 10.1126/science.276.5313.812

実験手法

X-RAY DIFFRACTION (2.6 Å)