1AIE

P53 TETRAMERIZATION DOMAIN CRYSTAL STRUCTURE

1AIE の概要

• エントリーDOI: 10.2210/pdb1aie/pdb
• 分子名称: P53 (2 entities in total)
• 機能のキーワード: p53 tetramerization, oligomer, dna, transcription, tumor suppressor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm. Isoform 1: Nucleus. Isoform 2: Nucleus. Isoform 3: Nucleus. Isoform 4: Nucleus. Isoform 7: Nucleus. Isoform 8: Nucleus. Isoform 9: Cytoplasm: P04637
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3766.22

構造登録者

Mittl, P.R.E.,Chene, P.,Gruetter, M.G. (登録日: 1997-04-17, 公開日: 1997-06-16, 最終更新日: 2024-05-22)

主引用文献

Mittl, P.R.,Chene, P.,Grutter, M.G.
Crystallization and structure solution of p53 (residues 326-356) by molecular replacement using an NMR model as template.
Acta Crystallogr.,Sect.D, 54:86-89, 1998

PubMed Abstract: The molecular replacement method is a powerful technique for crystal structure solution but the use of NMR structures as templates often causes problems. In this work the NMR structure of the p53 tetramerization domain has been used to solve the crystal structure by molecular replacement. Since the rotation- and translation-functions were not sufficiently clear, additional information about the symmetry of the crystal and the protein complex was used to identify correct solutions. The three-dimensional structure of residues 326-356 was subsequently refined to a final R factor of 19.1% at 1.5 A resolution.

PubMed: 9761820
DOI: 10.1107/S0907444997006550

実験手法

X-RAY DIFFRACTION (1.5 Å)