1AHO

THE AB INITIO STRUCTURE DETERMINATION AND REFINEMENT OF A SCORPION PROTEIN TOXIN

Summary for 1AHO

• Entry DOI: 10.2210/pdb1aho/pdb
• Descriptor: TOXIN II (2 entities in total)
• Functional Keywords: toxin ii, scorpion, ab initio phasing, neurotoxin
• Biological source: Androctonus australis
• Total number of polymer chains: 1
• Total formula weight: 7262.17

Authors

Smith, G.D.,Blessing, R.H.,Ealick, S.E.,Fontecilla-Camps, J.C.,Hauptman, H.A.,Housset, D.,Langs, D.A.,Miller, R. (deposition date: 1997-04-08, release date: 1997-10-15, Last modification date: 2024-10-30)

Primary citation

Smith, G.D.,Blessing, R.H.,Ealick, S.E.,Fontecilla-Camps, J.C.,Hauptman, H.A.,Housset, D.,Langs, D.A.,Miller, R.
Ab initio structure determination and refinement of a scorpion protein toxin.
Acta Crystallogr.,Sect.D, 53:551-557, 1997

PubMed Abstract: The structure of toxin II from the scorpion Androctonus australis Hector has been determined ab initio by direct methods using SnB at 0.96 A resolution. For the purpose of this structure redetermination, undertaken as a test of the minimal function and the SnB program, the identity and sequence of the protein was withheld from part of the research team. A single solution obtained from 1 619 random atom trials was clearly revealed by the bimodal distribution of the final value of the minimal function associated with each individual trial. Five peptide fragments were identified from a conservative analysis of the initial E-map, and following several refinement cycles with X-PLOR, a model was built of the complete structure. At the end of the X-PLOR refinement, the sequence was compared with the published sequence and 57 of the 64 residues had been correctly identified. Two errors in sequence resulted from side chains with similar size while the rest of the errors were a result of severe disorder or high thermal motion in the side chains. Given the amino-acid sequence, it is estimated that the initial E-map could have produced a model containing 99% of all main-chain and 81% of side-chain atoms. The structure refinement was completed with PROFFT, including the contributions of protein H atoms, and converged at a residual of 0.158 for 30 609 data with F >or= 2sigma(F) in the resolution range 8.0-0.964 A. The final model consisted of 518 non-H protein atoms (36 disordered), 407 H atoms, and 129 water molecules (43 with occupancies less than unity). This total of 647 non-H atoms represents the largest light-atom structure solved to date.

PubMed: 15299886
DOI: 10.1107/S0907444997005386

Experimental method

X-RAY DIFFRACTION (0.96 Å)