1AHK

DER F 2, THE MAJOR MITE ALLERGEN FROM DERMATOPHAGOIDES FARINAE, NMR, MINIMIZED AVERAGE STRUCTURE

1AHK の概要

• エントリーDOI: 10.2210/pdb1ahk/pdb
• 分子名称: DER F 2 (1 entity in total)
• 機能のキーワード: allergen, immunoglobulin fold
• 由来する生物種: Dermatophagoides farinae (American house dust mite)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14064.19

構造登録者

Ichikawa, S.,Hatanaka, H.,Yuuki, T.,Iwamoto, N.,Ogura, K.,Okumura, Y.,Inagaki, F. (登録日: 1997-04-07, 公開日: 1998-04-08, 最終更新日: 2024-10-30)

主引用文献

Ichikawa, S.,Hatanaka, H.,Yuuki, T.,Iwamoto, N.,Kojima, S.,Nishiyama, C.,Ogura, K.,Okumura, Y.,Inagaki, F.
Solution structure of Der f 2, the major mite allergen for atopic diseases.
J.Biol.Chem., 273:356-360, 1998

PubMed Abstract: House dust mites cause heavy atopic diseases such as asthma and dermatitis. Among allergens from Dermatophagoides farinae, Der f 2 shows the highest positive rate for atopic patients, but its biological function in mites has been perfectly unknown, as well as the functions of its homologs in human and other animals. We have determined the tertiary structure of Der f 2 by multidimensional nuclear magnetic resonance spectroscopy. Der f 2 was found to be a single-domain protein of immunoglobulin fold, and its structure was the most similar to those of the two regulatory domains of transglutaminase. This fact, binding to the bacterial surface, and other small pieces of information hinted that Der f 2 is related to the innate antibacterial defense system in mites. The immunoglobulin E epitopes are also discussed on the basis of the tertiary structure.

PubMed: 9417088
DOI: 10.1074/jbc.273.1.356

実験手法

SOLUTION NMR