1AFX

UGAA EUKARYOTIC RIBOSOMAL RNA TETRALOOP, NMR, 13 STRUCTURES

Summary for 1AFX

• Entry DOI: 10.2210/pdb1afx/pdb
• Descriptor: RNA (5'-R(*GP*GP*UP*GP*UP*GP*AP*AP*CP*AP*CP*C)-3') (1 entity in total)
• Functional Keywords: ribonucleic acid, tetraloop, ribosomal rna, rna
• Total number of polymer chains: 1
• Total formula weight: 3851.36

Authors

Butcher, S.E.,Dieckmann, T.,Feigon, J. (deposition date: 1997-03-15, release date: 1997-05-15, Last modification date: 2024-05-22)

Primary citation

Butcher, S.E.,Allain, F.H.,Feigon, J.
Solution structure of the loop B domain from the hairpin ribozyme.
Nat.Struct.Biol., 6:212-216, 1999

PubMed Abstract: The hairpin ribozyme is a small catalytic RNA with a unique two-domain structure. Here we present the solution structure of the loop B domain of the hairpin ribozyme, which contains most of the catalytically essential nucleotides. The 38-nucleotide domain contains a 16-nucleotide internal loop that forms one of the largest non-Watson-Crick segments of base pairing thus far determined by either NMR or crystallography. Since the solution structure of the smaller loop A domain has been previously solved, an NMR structure-based model of the 22,000 Mr hairpin ribozyme-substrate open complex emerges by joining the two domain structures. Strikingly, catalytically essential functional groups for the loop B domain are concentrated within an expanded minor groove, presenting a clear docking surface for the loop A domain.

PubMed: 10074938
DOI: 10.1038/6651

Experimental method

SOLUTION NMR