1AFF

DNA QUADRUPLEX CONTAINING GGGG TETRADS AND (T.A).A TRIADS, NMR, 8 STRUCTURES

1AFF の概要

• エントリーDOI: 10.2210/pdb1aff/pdb
• 分子名称: QUADRUPLEX DNA (5'-D(TP*AP*GP*G)-3') (1 entity in total)
• 機能のキーワード: dna-quadruplex, (t.a).a base triad, bombyx mori telomerique single repeat, t-a platform, deoxyribonucleic acid, dna
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 4923.42

構造登録者

Kettani, A.,Bouaziz, S.,Wang, W.,Jones, R.A.,Patel, D.J. (登録日: 1997-03-06, 公開日: 1997-08-20, 最終更新日: 2024-05-22)

主引用文献

Kettani, A.,Bouaziz, S.,Wang, W.,Jones, R.A.,Patel, D.J.
Bombyx mori single repeat telomeric DNA sequence forms a G-quadruplex capped by base triads.
Nat.Struct.Biol., 4:382-389, 1997

PubMed Abstract: A combined NMR-molecular dynamics approach has been applied to determine the solution structure of a truncated analogue of the Bombyx mori telomeric d(TTAGG) single repeat sequence in Na+ cation-containing aqueous solution. The two-fold symmetric four-stranded d(TAGG) quadruplex contains two adjacent G(syn).G(syn).G(anti).G(anti) G-tetrads sandwiched between novel (T.A).A triads with individual strands having both a parallel and antiparallel neighbour around the quadruplex. The (T.A).A triad represents the first experimental verification of a base triad alignment which constitutes a key postulate in the recently proposed model of triad-DNA. Further, the (T.A).A triad is generated by positioning an A residue through hydrogen bonding in the minor groove of a Watson-Crick T.A base pair and includes a T-A platform related to an A-A platform recently observed in the structure of the P4-P6 domain of the Tetrahymena self splicing group I ribozyme. The novel architecture of the truncated Bombyx mori quadruplex structure sets the stage for the design and potential identification of additional base tetrads and triads that could participate in pairing alignments of multi-stranded DNA structures during chromosome association and genetic recombination.

PubMed: 9145109
DOI: 10.1038/nsb0597-382

実験手法

SOLUTION NMR