1AE7

NOTEXIN, A PRESYNAPTIC NEUROTOXIC PHOSPHOLIPASE A2

1AE7 の概要

• エントリーDOI: 10.2210/pdb1ae7/pdb
• 分子名称: PHOSPHOLIPASE A2, SULFATE ION (3 entities in total)
• 機能のキーワード: hydrolase, phospholipase a2, lipid degradation, presynaptic neurotoxin, venom
• 由来する生物種: Notechis scutatus scutatus
• 細胞内の位置: Secreted: P00608
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13900.68

構造登録者

Westerlund, B.,Nordlund, P.,Uhlin, U.,Eaker, D.,Eklund, H. (登録日: 1997-03-06, 公開日: 1997-05-15, 最終更新日: 2024-10-09)

主引用文献

Westerlund, B.,Nordlund, P.,Uhlin, U.,Eaker, D.,Eklund, H.
The three-dimensional structure of notexin, a presynaptic neurotoxic phospholipase A2 at 2.0 A resolution.
FEBS Lett., 301:159-164, 1992

PubMed Abstract: The three-dimensional structure of notexin has been solved by molecular replacement methods. The structure has been refined at 2.0 A resolution to a crystallographic R-value of 16.5% with good stereo-chemistry. The core of the protein is very similar to other phospholipase A2s (PLA2 s) but several parts of the molecule are distinctly different. The most significant differences from PLA2 s from bovine pancreas and rattlesnake occur in the stretches 56-80 and 85-89. Residue 69, which has been shown to be important for phospholipase binding, has a different conformation and different interactions than in other known PLA2s. The C alpha positions for residues 86-88 differ by about 6 A from both the bovine and the rattlesnake enzyme. The crystals contain no Ca2+ ions. Instead, a water molecule occupies the calcium site.

PubMed: 1568473
DOI: 10.1016/0014-5793(92)81238-H

実験手法

X-RAY DIFFRACTION (2 Å)