1ADD

A PRE-TRANSITION STATE MIMIC OF AN ENZYME: X-RAY STRUCTURE OF ADENOSINE DEAMINASE WITH BOUND 1-DEAZA-ADENOSINE AND ZINC-ACTIVATED WATER

1ADD の概要

• エントリーDOI: 10.2210/pdb1add/pdb
• 分子名称: ADENOSINE DEAMINASE, ZINC ION, 1-DEAZA-ADENOSINE, ... (4 entities in total)
• 機能のキーワード: hydrolase(acting in cyclicamidines)
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Cell membrane; Peripheral membrane protein; Extracellular side (By similarity): P03958
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40046.85

構造登録者

Wilson, D.K.,Quiocho, F.A. (登録日: 1992-12-22, 公開日: 1994-01-31, 最終更新日: 2024-02-07)

主引用文献

Wilson, D.K.,Quiocho, F.A.
A pre-transition-state mimic of an enzyme: X-ray structure of adenosine deaminase with bound 1-deazaadenosine and zinc-activated water.
Biochemistry, 32:1689-1694, 1993

PubMed Abstract: The refined 2.4-A structure of adenosine deaminase, recently discovered to be a zinc metalloenzyme [Wilson, D. K., Rudolph, F. B., & Quiocho, F. A. (1991) Science 252, 1278-1284], complexed with the ground-state analog 1-deazaadenosine shows the mode of binding of the analog and, unexpectedly, a zinc-activated water (hydroxide). This structure of a pre-transition-state mimic, combined with that previously determined for the complex with 6(R)-hydroxy-1,6-dihydropurine ribonucleoside, a nearly ideal transition-state analog, sheds new understanding of the precise stereospecificity and hydrolytic catalysis of an important and well-characterized member of a large group of zinc metalloenzymes. As both of these excellent mimics were generated in the active site, they demonstrate a powerful means of dissecting the course of an enzymatic reaction by direct crystallographic analysis.

PubMed: 8439534
DOI: 10.1021/bi00058a001

実験手法

X-RAY DIFFRACTION (2.4 Å)