1ACZ

GLUCOAMYLASE, GRANULAR STARCH-BINDING DOMAIN COMPLEX WITH CYCLODEXTRIN, NMR, 5 STRUCTURES

1ACZ の概要

• エントリーDOI: 10.2210/pdb1acz/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900012
• 分子名称: GLUCOAMYLASE, Cycloheptakis-(1-4)-(alpha-D-glucopyranose) (2 entities in total)
• 機能のキーワード: hydrolase, starch binding domain, glycosidase, polysaccharide degradation, glycoprotein, alternative splicing
• 由来する生物種: Aspergillus niger
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14190.82

構造登録者

Sorimachi, K.,Le Gal-Coeffet, M.-F.,Williamson, G.,Archer, D.B.,Williamson, M.P. (登録日: 1997-02-10, 公開日: 1997-07-07, 最終更新日: 2024-11-06)

主引用文献

Sorimachi, K.,Le Gal-Coeffet, M.F.,Williamson, G.,Archer, D.B.,Williamson, M.P.
Solution structure of the granular starch binding domain of Aspergillus niger glucoamylase bound to beta-cyclodextrin.
Structure, 5:647-661, 1997

PubMed Abstract: Carbohydrate-binding domains are usually small and physically separate from the catalytic domains of hydrolytic enzymes. Glucoamylase 1 (G1) from Aspergillus niger, an enzyme used widely in the food and brewing industries, contains a granular starch binding domain (SBD) which is separated from the catalytic domain by a semi-rigid linker. The aim of this study was to determine how the SBD binds to starch, and thereby more generally to throw light on the role of carbohydrate-binding domains in the hydrolysis of insoluble polysaccharides.

PubMed: 9195884
DOI: 10.1016/S0969-2126(97)00220-7

実験手法

SOLUTION NMR