1AA6

REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI

1AA6 の概要

• エントリーDOI: 10.2210/pdb1aa6/pdb
• 分子名称: FORMATE DEHYDROGENASE H, IRON/SULFUR CLUSTER, 2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE GUANOSINE DINUCLEOTIDE, ... (5 entities in total)
• 機能のキーワード: oxidoreductase, selenium, selenocysteine, secys, molybdenum, molybdopterin, mpt, molybdopterin guanine dinucleotide, mgd, iron sulfur cluster, fe4s4, formate, dehydrogenase, anaerobic
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 81394.40

構造登録者

Sun, P.D.,Boyington, J.C. (登録日: 1997-01-23, 公開日: 1997-08-20, 最終更新日: 2024-02-07)

主引用文献

Boyington, J.C.,Gladyshev, V.N.,Khangulov, S.V.,Stadtman, T.C.,Sun, P.D.
Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster.
Science, 275:1305-1308, 1997

PubMed Abstract: Formate dehydrogenase H from Escherichia coli contains selenocysteine (SeCys), molybdenum, two molybdopterin guanine dinucleotide (MGD) cofactors, and an Fe4S4 cluster at the active site and catalyzes the two-electron oxidation of formate to carbon dioxide. The crystal structures of the oxidized [Mo(VI), Fe4S4(ox)] form of formate dehydrogenase H (with and without bound inhibitor) and the reduced [Mo(IV), Fe4S4(red)] form have been determined, revealing a four-domain alphabeta structure with the molybdenum directly coordinated to selenium and both MGD cofactors. These structures suggest a reaction mechanism that directly involves SeCys140 and His141 in proton abstraction and the molybdenum, molybdopterin, Lys44, and the Fe4S4 cluster in electron transfer.

PubMed: 9036855
DOI: 10.1126/science.275.5304.1305

実験手法

X-RAY DIFFRACTION (2.3 Å)