1AA3

C-TERMINAL DOMAIN OF THE E. COLI RECA, NMR, MINIMIZED AVERAGE STRUCTURE

1AA3 の概要

• エントリーDOI: 10.2210/pdb1aa3/pdb
• 分子名称: RECA (1 entity in total)
• 機能のキーワード: double-stranded dna binding domain, riken structural genomics/proteomics initiative, rsgi, structural genomics
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: P0A7G6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7108.13

構造登録者

Aihara, H.,Ito, Y.,Kurumizaka, H.,Terada, T.,Yokoyama, S.,Shibata, T.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 1997-01-22, 公開日: 1997-07-23, 最終更新日: 2024-04-10)

主引用文献

Aihara, H.,Ito, Y.,Kurumizaka, H.,Terada, T.,Yokoyama, S.,Shibata, T.
An interaction between a specified surface of the C-terminal domain of RecA protein and double-stranded DNA for homologous pairing.
J.Mol.Biol., 274:213-221, 1997

PubMed Abstract: RecA protein and its homologs catalyze homologous pairing of dsDNA and ssDNA, a critical reaction in homologous genetic recombination in various organisms from a virus, microbes to higher eukaryotes. In this reaction, RecA protein forms a nucleoprotein filament on ssDNA, which in turn binds to naked dsDNA for homology search. We suggested that the C-terminal domain of RecA protein plays a role in capturing the dsDNA. Here, we isolated the C-terminal domain as a soluble form and determined the solution structure by NMR spectroscopy. The overall folding of the NMR structure agrees with that of the corresponding part of the reported crystal structure, but a remarkable difference was found in a solvent-exposed region due to intermolecular contacts in the crystal. Then, we studied the interaction between the C-terminal domain and DNA, and found that significant chemical shift changes were induced in a specific region by titration with dsDNA. SsDNA induced a much smaller chemical shift perturbation. The difference of DNA concentrations to give the half-saturation of the chemical shift change showed a higher affinity of the C-terminal region toward dsDNA. Combined with our previous results, these provide direct evidence that the defined region in the C-terminal domain furnishes a binding surface for DNA.

PubMed: 9398528
DOI: 10.1006/jmbi.1997.1403

実験手法

SOLUTION NMR