1A9J

APYRIMIDINIC DNA WITH BOUND WATER AT THE DAMAGED SITE, BETA FORM, NMR, 1 STRUCTURE

1A9J の概要

• エントリーDOI: 10.2210/pdb1a9j/pdb
• 分子名称: DNA (5'-D(*CP*GP*CP*GP*AP*AABP*AP*CP*GP*CP*C)-3'), DNA (5'-D(*GP*GP*CP*GP*TP*AP*TP*CP*GP*CP*G)-3') (3 entities in total)
• 機能のキーワード: damaged dna, apyrimidinic dna, water, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6601.29

構造登録者

Beger, R.D.,Bolton, P.H. (登録日: 1998-04-06, 公開日: 1998-07-15, 最終更新日: 2024-04-10)

主引用文献

Beger, R.D.,Bolton, P.H.
Structures of apurinic and apyrimidinic sites in duplex DNAs.
J.Biol.Chem., 273:15565-15573, 1998

PubMed Abstract: Natural and exogenous processes can give rise to abasic sites with either a purine or pyrimidine as the base on the opposing strand. The solution state structures of the apyrimidinic DNA duplex, with D6 indicating an abasic site, [sequence: see text] referred to as AD, and the apurinic DNA duplex with a dC17, referred to as CD, have been determined. A particularly striking difference is that the abasic site in CD is predominantly a beta hemiacetal, whereas in AD the alpha and beta forms are equally present. Hydrogen bonding with water by the abasic site and the base on the opposite strand appears to play a large role in determining the structure near the damaged site. Comparison of these structures with that of a duplex DNA containing a thymine glycol at the same position as the abasic site and with that of a duplex DNA containing an abasic site in the middle of a curved DNA sequence offers some insight into the common and distinct structural features of damaged DNA sites.

PubMed: 9624147
DOI: 10.1074/jbc.273.25.15565

実験手法

SOLUTION NMR