1A7U

CHLOROPEROXIDASE T

1A7U の概要

• エントリーDOI: 10.2210/pdb1a7u/pdb
• 分子名称: CHLOROPEROXIDASE T (2 entities in total)
• 機能のキーワード: haloperoxidase, oxidoreductase, peroxidase, alpha/beta hydrolase fold
• 由来する生物種: Streptomyces aureofaciens
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 60498.88

構造登録者

Hofmann, B.,Toelzer, S.,Pelletier, I.,Altenbuchner, J.,Van Pee, K.-H.,Hecht, H.-J. (登録日: 1998-03-17, 公開日: 1998-06-17, 最終更新日: 2024-05-22)

主引用文献

Hofmann, B.,Tolzer, S.,Pelletier, I.,Altenbuchner, J.,van Pee, K.H.,Hecht, H.J.
Structural investigation of the cofactor-free chloroperoxidases.
J.Mol.Biol., 279:889-900, 1998

PubMed Abstract: The structures of cofactor-free haloperoxidases from Streptomyces aureofaciens, Streptomyces lividans, and Pseudomonas fluorescens have been determined at resolutions between 1.9 A and 1.5 A. The structures of two enzymes complexed with benzoate or propionate identify the binding site for the organic acids which are required for the haloperoxidase activity. Based on these complexes and on the structure of an inactive variant, a reaction mechanism is proposed for the halogenation reaction with peroxoacid and hypohalous acid as reaction intermediates. Comparison of the structures suggests that a specific halide binding site is absent in the enzymes but that hydrophobic organic compounds may fit into the active site pocket for halogenation at preferential sites.

PubMed: 9642069
DOI: 10.1006/jmbi.1998.1802

実験手法

X-RAY DIFFRACTION (1.75 Å)