1A7M

LEUKAEMIA INHIBITORY FACTOR CHIMERA (MH35-LIF), NMR, 20 STRUCTURES

1A7M の概要

• エントリーDOI: 10.2210/pdb1a7m/pdb
• 分子名称: LEUKEMIA INHIBITORY FACTOR (1 entity in total)
• 機能のキーワード: cytokine, four helical bundle
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19822.94

構造登録者

Hinds, M.G.,Maurer, T.,Zhang, J.-G.,Nicola, N.A.,Norton, R.S. (登録日: 1998-03-16, 公開日: 1999-04-20, 最終更新日: 2024-10-16)

主引用文献

Hinds, M.G.,Maurer, T.,Zhang, J.G.,Nicola, N.A.,Norton, R.S.
Solution structure of leukemia inhibitory factor.
J.Biol.Chem., 273:13738-13745, 1998

PubMed Abstract: The solution structure of a murine-human chimera of leukemia inhibitory factor (LIF), a 180-residue cytokine with a molecular mass of 20 kDa, has been determined using multidimensional heteronuclear NMR techniques. The protein contains four alpha-helices, the relative orientations of which are well defined on the basis of long-range interhelical nuclear Overhauser effects. The helices are arranged in an up-up-down-down orientation, as found in other four-helix bundle cytokines, and the overall topology of the chimera is similar to that of the crystal structure of murine LIF (Robinson, R. C., Grey, L. M., Staunton, D., Vankelecom, H. Vernallis, A. B., Moreau, J. F., Stuart, D. I., Heath, J. K., and Jones, E. Y. (1994) Cell 77, 1101-1116). Differences between the structures are evident in the N-terminal region, where the peptide bond preceding Pro17 has a trans-conformation in solution but a cis-conformation in the crystal, and in the small antiparallel beta-sheet encompassing residues in the N terminus and the CD loop in the crystal structure, which is not apparent in solution. There are also minor differences in the extent of the helices. Other than at the N terminus, the main difference between the two structures occurs at the C-terminal end of the CD loop. As this loop is close to a receptor-binding site on LIF that makes a major contribution to high affinity binding to the LIF receptor alpha-chain, these differences between the solution and crystal structures should be taken into account in structural models of LIF receptor interactions.

PubMed: 9593715
DOI: 10.1074/jbc.273.22.13738

実験手法

SOLUTION NMR