1A79

CRYSTAL STRUCTURE OF THE TRNA SPLICING ENDONUCLEASE FROM METHANOCOCCUS JANNASCHII

Summary for 1A79

Entry DOI	10.2210/pdb1a79/pdb
Descriptor	TRNA ENDONUCLEASE, SULFATE ION, GOLD ION, ... (4 entities in total)
Functional Keywords	endonuclease, trna endonuclease
Biological source	Methanocaldococcus jannaschii
Total number of polymer chains	4
Total formula weight	79963.13
Authors	Li, H.,Trotta, C.R.,Abelson, J.N. (deposition date: 1998-03-23, release date: 1999-06-01, Last modification date: 2024-02-07)
Primary citation	Li, H.,Trotta, C.R.,Abelson, J. Crystal structure and evolution of a transfer RNA splicing enzyme. Science, 280:279-284, 1998 Cited by PubMed Abstract: The splicing of transfer RNA precursors is similar in Eucarya and Archaea. In both kingdoms an endonuclease recognizes the splice sites and releases the intron, but the mechanism of splice site recognition is different in each kingdom. The crystal structure of the endonuclease from the archaeon Methanococcus jannaschii was determined to a resolution of 2.3 angstroms. The structure indicates that the cleavage reaction is similar to that of ribonuclease A and the arrangement of the active sites is conserved between the archaeal and eucaryal enzymes. These results suggest an evolutionary pathway for splice site recognition. PubMed: 9535656 DOI: 10.1126/science.280.5361.279 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.28 Å)

Structure validation