1A79

CRYSTAL STRUCTURE OF THE TRNA SPLICING ENDONUCLEASE FROM METHANOCOCCUS JANNASCHII

1A79 の概要

• エントリーDOI: 10.2210/pdb1a79/pdb
• 分子名称: TRNA ENDONUCLEASE, SULFATE ION, GOLD ION, ... (4 entities in total)
• 機能のキーワード: endonuclease, trna endonuclease
• 由来する生物種: Methanocaldococcus jannaschii
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 79963.13

構造登録者

Li, H.,Trotta, C.R.,Abelson, J.N. (登録日: 1998-03-23, 公開日: 1999-06-01, 最終更新日: 2024-02-07)

主引用文献

Li, H.,Trotta, C.R.,Abelson, J.
Crystal structure and evolution of a transfer RNA splicing enzyme.
Science, 280:279-284, 1998

PubMed Abstract: The splicing of transfer RNA precursors is similar in Eucarya and Archaea. In both kingdoms an endonuclease recognizes the splice sites and releases the intron, but the mechanism of splice site recognition is different in each kingdom. The crystal structure of the endonuclease from the archaeon Methanococcus jannaschii was determined to a resolution of 2.3 angstroms. The structure indicates that the cleavage reaction is similar to that of ribonuclease A and the arrangement of the active sites is conserved between the archaeal and eucaryal enzymes. These results suggest an evolutionary pathway for splice site recognition.

PubMed: 9535656
DOI: 10.1126/science.280.5361.279

実験手法

X-RAY DIFFRACTION (2.28 Å)