1A6Y

REVERBA ORPHAN NUCLEAR RECEPTOR/DNA COMPLEX

Summary for 1A6Y

• Entry DOI: 10.2210/pdb1a6y/pdb
• Descriptor: DNA (5'-D(*CP*AP*AP*CP*TP*AP*GP*GP*TP*CP*AP*CP*(5IT)P*AP*GP*GP*TP*CP*AP*G)-3'), DNA (5'-D(*CP*TP*GP*AP*CP*CP*TP*AP*GP*TP*GP*AP*CP*CP*TP*AP*GP*TP*TP*G)-3'), ORPHAN NUCLEAR RECEPTOR NR1D1, ... (5 entities in total)
• Functional Keywords: orphan receptor, nuclear receptor, dna-binding, reverb, rev-erb, transcription regulation, transcription-dna complex, transcription/dna
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus: P20393
• Total number of polymer chains: 4
• Total formula weight: 34445.48

Authors

Zhao, Q.,Khorasanizadeh, S.,Rastinejad, F. (deposition date: 1998-03-04, release date: 1998-10-21, Last modification date: 2023-08-02)

Primary citation

Zhao, Q.,Khorasanizadeh, S.,Miyoshi, Y.,Lazar, M.A.,Rastinejad, F.
Structural elements of an orphan nuclear receptor-DNA complex.
Mol.Cell, 1:849-861, 1998

PubMed Abstract: The nuclear hormone receptors form the largest known family of transcription factors. The current notion of receptor DNA discrimination, based solely on one major type of hexameric half-site and a highly conserved 66-residue core DNA-binding domain (DBD), does not adequately describe how more than 150 nonsteroid receptors differentiate among response elements. Here, we describe the 2.3 A crystal structure of the DNA-binding region of the orphan receptor RevErb arranged as a tandem homodimer on its optimal response element. The structure reveals the presence of a second major protein-DNA interface adjacent to the classical one involving the half-sites. A sequence comparison of orphan receptors suggests that unique minor-groove interactions involving the receptor hinge regions impart the necessary DNA and dimerization specificity.

PubMed: 9660968
DOI: 10.1016/S1097-2765(00)80084-2

Experimental method

X-RAY DIFFRACTION (2.3 Å)