1A6J

NITROGEN REGULATORY BACTERIAL PROTEIN IIA-NITROGEN

1A6J の概要

• エントリーDOI: 10.2210/pdb1a6j/pdb
• 分子名称: NITROGEN REGULATORY IIA PROTEIN, BETA-MERCAPTOETHANOL, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: phosphotransferase system, nitrogen regulation
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: P69829
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36215.41

構造登録者

Bordo, D.,Van Montfort, R.,Pijning, T.,Kalk, K.H.,Reizer, J.,Saier, M.H.,Dijkstra, B.W. (登録日: 1998-02-25, 公開日: 1998-07-15, 最終更新日: 2024-02-07)

主引用文献

Bordo, D.,van Monfort, R.L.,Pijning, T.,Kalk, K.H.,Reizer, J.,Saier Jr., M.H.,Dijkstra, B.W.
The three-dimensional structure of the nitrogen regulatory protein IIANtr from Escherichia coli.
J.Mol.Biol., 279:245-255, 1998

PubMed Abstract: The bacterial rpoN operon codes for sigma 54, which is the key sigma factor that, under nitrogen starvation conditions, activates the transcription of genes needed to assimilate ammonia and glutamate. The rpoN operon contains several other open reading frames that are cotranscribed with sigma 54. The product of one of these, the 17.9 kDa protein IIANtr, is homologous to IIA proteins of the phosphoenolpyruvate:sugar phosphotransferase (PTS) system. IIANtr influences the transcription of sigma 54-dependent genes through an unknown mechanism and may thereby provide a regulatory link between carbon and nitrogen metabolism. Here we describe the 2.35 A X-ray structure of Escherichia coli IIANtr. It is the first structure of a IIA enzyme from the fructose-mannitol family of the PTS. The enzyme displays a novel fold characterized by a central mixed parallel/anti-parallel beta-sheet surrounded by six alpha-helices. The active site His73 is situated in a shallow depression on the protein surface.

PubMed: 9636714
DOI: 10.1006/jmbi.1998.1753

実験手法

X-RAY DIFFRACTION (2.35 Å)