1A6I

TET REPRESSOR, CLASS D VARIANT

1A6I の概要

• エントリーDOI: 10.2210/pdb1a6i/pdb
• 分子名称: TETRACYCLINE REPRESSOR PROTEIN CLASS D (2 entities in total)
• 機能のキーワード: transcription regulation, repressor, dna-binding
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24319.69

構造登録者

Orth, P.,Cordes, F.,Schnappinger, D.,Hillen, W.,Saenger, W.,Hinrichs, W. (登録日: 1998-02-25, 公開日: 1999-03-02, 最終更新日: 2024-05-22)

主引用文献

Orth, P.,Cordes, F.,Schnappinger, D.,Hillen, W.,Saenger, W.,Hinrichs, W.
Conformational changes of the Tet repressor induced by tetracycline trapping.
J.Mol.Biol., 279:439-447, 1998

PubMed Abstract: The X-ray crystal structure analysis of inducer-free Tet repressor, TetR, at 2.4 A resolution identifies one of two openings of the tunnel-like binding site as the entrance for the inducer tetracycline-Mg2+, [Mg Tc]+. Recognition and binding of the inducer unleashes conformational changes leading to the induced state of TetR. In the first step, the C-terminal turn of alpha-helix 6 unwinds, thereby altering the orientation of alpha-helix 4. This different orientation of alpha-helix 4 is stabilized by a series of hydrogen bonds mediated through a chain of eight water molecules. The alpha-helix 4 connects the DNA-binding domain (alpha-helices 1 to 3) to the rigid TetR core, and thus regulates gene expression through its respective orientations.

PubMed: 9642048
DOI: 10.1006/jmbi.1998.1775

実験手法

X-RAY DIFFRACTION (2.4 Å)