1A4J

DIELS ALDER CATALYTIC ANTIBODY GERMLINE PRECURSOR

1A4J の概要

• エントリーDOI: 10.2210/pdb1a4j/pdb
• 分子名称: IMMUNOGLOBULIN, DIELS ALDER CATALYTIC ANTIBODY (LIGHT CHAIN), IMMUNOGLOBULIN, DIELS ALDER CATALYTIC ANTIBODY (HEAVY CHAIN) (3 entities in total)
• 機能のキーワード: immunoglobulin, antibody, catalytic antibody, diels alder, germline
• 由来する生物種: Mus musculus (house mouse); 詳細
• 細胞内の位置: Secreted : P01834
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 94599.87

構造登録者

Spiller, B.W.,Romesburg, F.E.,Schultz, P.G.,Stevens, R.C. (登録日: 1998-01-30, 公開日: 1998-05-13, 最終更新日: 2024-10-30)

主引用文献

Romesberg, F.E.,Spiller, B.,Schultz, P.G.,Stevens, R.C.
Immunological origins of binding and catalysis in a Diels-Alderase antibody.
Science, 279:1929-1933, 1998

PubMed Abstract: The three-dimensional structure of an antibody (39-A11) that catalyzes a Diels-Alder reaction has been determined. The structure suggests that the antibody catalyzes this pericyclic reaction through a combination of packing and hydrogen-bonding interactions that control the relative geometries of the bound substrates and electronic distribution in the dienophile. A single somatic mutation, serine-91 of the light chain to valine, is largely responsible for the increase in affinity and catalytic activity of the affinity-matured antibody. Structural and functional studies of the germ-line precursor suggest that 39-A11 and related antibodies derive from a family of germ-line genes that have been selected throughout evolution for the ability of the encoded proteins to form a polyspecific combining site. Germ line-encoded antibodies of this type, which can rapidly evolve into high-affinity receptors for a broad range of structures, may help to expand the binding potential associated with the structural diversity of the primary antibody repertoire.

PubMed: 9506942
DOI: 10.1126/science.279.5358.1929

実験手法

X-RAY DIFFRACTION (2.1 Å)