1A0P

SITE-SPECIFIC RECOMBINASE, XERD

1A0P の概要

• エントリーDOI: 10.2210/pdb1a0p/pdb
• 分子名称: SITE-SPECIFIC RECOMBINASE XERD (2 entities in total)
• 機能のキーワード: xerd, recombinase, dna binding, dna recombination
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm (By similarity): P0A8P8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33308.19

構造登録者

Subramanya, H.S.,Arciszewska, L.K.,Baker, R.A.,Bird, L.E.,Sherratt, D.J.,Wigley, D.B. (登録日: 1997-12-05, 公開日: 1998-03-18, 最終更新日: 2024-02-07)

主引用文献

Subramanya, H.S.,Arciszewska, L.K.,Baker, R.A.,Bird, L.E.,Sherratt, D.J.,Wigley, D.B.
Crystal structure of the site-specific recombinase, XerD.
EMBO J., 16:5178-5187, 1997

PubMed Abstract: The structure of the site-specific recombinase, XerD, that functions in circular chromosome separation, has been solved at 2.5 A resolution and reveals that the protein comprises two domains. The C-terminal domain contains two conserved sequence motifs that are located in similar positions in the structures of XerD, lambda and HP1 integrases. However, the extreme C-terminal regions of the three proteins, containing the active site tyrosine, are very different. In XerD, the arrangement of active site residues supports a cis cleavage mechanism. Biochemical evidence for DNA bending is encompassed in a model that accommodates extensive biochemical and genetic data, and in which the DNA is wrapped around an alpha-helix in a manner similar to that observed for CAP complexed with DNA.

PubMed: 9311978
DOI: 10.1093/emboj/16.17.5178

実験手法

X-RAY DIFFRACTION (2.5 Å)