197D

ORTHORHOMBIC CRYSTAL STRUCTURE OF THE A-DNA OCTAMER D(GTACGTAC). COMPARISON WITH THE TETRAGONAL STRUCTURE

Summary for 197D

• Entry DOI: 10.2210/pdb197d/pdb
• Descriptor: DNA (5'-D(*GP*TP*AP*CP*GP*TP*AP*C)-3') (2 entities in total)
• Functional Keywords: a-dna, double helix, dna
• Total number of polymer chains: 2
• Total formula weight: 4853.23

Authors

Langlois D'Estaintot, B.,Dautant, A.,Courseille, C.,Precigoux, G. (deposition date: 1994-11-24, release date: 1995-02-07, Last modification date: 2024-02-07)

Primary citation

Langlois d'Estaintot, B.,Dautant, A.,Courseille, C.,Precigoux, G.
Orthorhombic crystal structure of the A-DNA octamer d(GTACGTAC). Comparison with the tetragonal structure.
Eur.J.Biochem., 213:673-682, 1993

PubMed Abstract: The X-ray crystal structure of the double-helical A-DNA octanucleotide d(GTACGTAC) has been solved by molecular replacement and refined to a resolution of 0.219 nm. The final R-factor is equal to 16.1% for 1516 observed reflections with F > 4 sigma(F). The sequence crystallizes as an A-DNA-type double helix in the orthorhombic space group P2(1)2(1)2, with one duplex molecule solvated by 66 water molecules in the asymmetric unit. Cell parameters are a = 3.860 nm, b = 5.082 nm, c = 2.174 nm. It is the first time that such a crystal form has been observed. This orthorhombic structure has been compared with the tetragonal structure of the same oligonucleotide. It adopts a bent structure with an unusual packing between symmetry-related molecules.

PubMed: 8477741
DOI: 10.1111/j.1432-1033.1993.tb17807.x

Experimental method

X-RAY DIFFRACTION (2.19 Å)