178D

CRYSTAL STRUCTURE OF A DNA DUPLEX CONTAINING 8-HYDROXYDEOXYGUANINE.ADENINE BASE-PAIRS

178D の概要

• エントリーDOI: 10.2210/pdb178d/pdb
• 分子名称: DNA (5'-D(*CP*GP*CP*AP*AP*AP*TP*TP*(8OG)P*GP*CP*G)-3') (2 entities in total)
• 機能のキーワード: b-dna, double helix, modified, mismatched, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7406.83

構造登録者

McAuley-Hecht, K.E.,Leonard, G.A.,Gibson, N.J.,Thomson, J.B.,Watson, W.P.,Hunter, W.N.,Brown, T. (登録日: 1994-05-19, 公開日: 1994-10-04, 最終更新日: 2024-02-07)

主引用文献

McAuley-Hecht, K.E.,Leonard, G.A.,Gibson, N.J.,Thomson, J.B.,Watson, W.P.,Hunter, W.N.,Brown, T.
Crystal structure of a DNA duplex containing 8-hydroxydeoxyguanine-adenine base pairs.
Biochemistry, 33:10266-10270, 1994

PubMed Abstract: The crystal structure of the oligonucleotide d(CGCAAATTO8GGCG), containing the chemically modified base 8-hydroxydeoxyguanine (O8G), has been determined at 2.5-A resolution and refined to a crystallographic R-factor of 16.8%. The B-type DNA helix contains standard Watson-Crick base pairs except at the mismatch sites, where O8G adopts a syn conformation and forms hydrogen bonds to adenine in the anti conformation. The thermodynamic stability of the duplex was found by UV melting techniques to be intermediate between the native oligonucleotide d(CGCAAATTTGCG) and an oligonucleotide containing A.G mispairs d(CGCAAATTGGCG). Comparison of the structure of the O8G(syn).A(anti) base pair with those of Watson-Crick base pairs has given a reason why O8G.A base pairs are not well repaired by DNA proofreading enzymes.

PubMed: 8068665
DOI: 10.1021/bi00200a006

実験手法

X-RAY DIFFRACTION (2.5 Å)