176D

NMR SOLUTION STRUCTURE OF A PEPTIDE NUCLEIC ACID COMPLEXED WITH RNA

176D の概要

• エントリーDOI: 10.2210/pdb176d/pdb
• 分子名称: DNA (5'-D(*GPN*APN*APN*CPN*TPN*CPN)-3'), RNA (5'-R(P*GP*AP*GP*UP*UP*C)-3') (2 entities in total)
• 機能のキーワード: rna, pna, double helix, peptide nucleic acid-rna complex, peptide nucleic acid/rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 3526.77

構造登録者

Brown, S.C.,Veal, J.M. (登録日: 1994-05-17, 公開日: 1994-11-01, 最終更新日: 2024-11-13)

主引用文献

Brown, S.C.,Thomson, S.A.,Veal, J.M.,Davis, D.G.
NMR solution structure of a peptide nucleic acid complexed with RNA.
Science, 265:777-780, 1994

PubMed Abstract: Peptide nucleic acids (PNA) incorporating nucleic acid bases into an achiral polyamide backbone bind to DNA in a sequence-dependent manner. The structure of a PNA-ribonucleic acid (RNA) complex was determined with nuclear magnetic resonance methods. A hexameric PNA formed a 1:1 complex with a complementary RNA that is an antiparallel, right-handed double helix with Watson-Crick base pairing similar to the "A" form structure of RNA duplexes. The achiral PNA backbone assumed a distinct conformation upon binding that differed from previously proposed models and provides a basis for further structure-based design of antisense agents.

PubMed: 7519361

実験手法

SOLUTION NMR