159D

SIDE BY SIDE BINDING OF TWO DISTAMYCIN A DRUGS IN THE MINOR GROOVE OF AN ALTERNATING B-DNA DUPLEX

159D の概要

• エントリーDOI: 10.2210/pdb159d/pdb
• 分子名称: DNA (5'-D(*IP*CP*IP*CP*IP*CP*IP*C)-3'), DISTAMYCIN A, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: b-dna, double helix, complexed with drug, modified, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2874.35

構造登録者

Chen, X.,Ramakrishnan, B.,Rao, S.T.,Sundaralingam, M. (登録日: 1994-02-10, 公開日: 1995-02-07, 最終更新日: 2024-02-07)

主引用文献

Chen, X.,Ramakrishnan, B.,Rao, S.T.,Sundaralingam, M.
Binding of two distamycin A molecules in the minor groove of an alternating B-DNA duplex.
Nat.Struct.Biol., 1:169-175, 1994

PubMed Abstract: Here we report the 1.8A X-ray structure of a 2:1 drug-DNA complex between distamycin A and an alternating B-DNA octamer duplex d(ICICICIC)2. The two distamycin A molecules are bound side by side with dyad symmetry in an antiparallel orientation in the expanded minor groove. The amides of each drug molecule are hydrogen bonded to the minor groove base atoms of only one DNA strand. The complex not only shows binding of two drug molecules, but the DNA duplex also exhibits striking low-high alternations in the helical twist angles, the sugar puckering and the phosphate conformations, providing the basis for a new model for an alternating B-DNA with a dinucleotide repeat.

PubMed: 7656035
DOI: 10.1038/nsb0394-169

実験手法

X-RAY DIFFRACTION (1.8 Å)