133D

THE CRYSTAL STRUCTURE OF N4-METHYLCYTOSINE.GUANOSIN BASE-PAIRS IN THE SYNTHETIC HEXANUCLEOTIDE D(CGCGM(4)CG)

133D の概要

• エントリーDOI: 10.2210/pdb133d/pdb
• 分子名称: DNA (5'-D(*CP*GP*CP*GP*(C34)P*G)-3') (2 entities in total)
• 機能のキーワード: z-dna, double helix, modified, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 3648.46

構造登録者

Cervi, A.R.,Guy, A.,Leonard, G.A.,Teoule, R.,Hunter, W.N. (登録日: 1993-07-29, 公開日: 1994-01-15, 最終更新日: 2024-02-07)

主引用文献

Cervi, A.R.,Guy, A.,Leonard, G.A.,Teoule, R.,Hunter, W.N.
The crystal structure of N4-methylcytosine.guanosine base-pairs in the synthetic hexanucleotide d(CGCGm4CG).
Nucleic Acids Res., 21:5623-5629, 1993

PubMed Abstract: The structure of d(CGCGm4CG) were m4C = N4-methylcytosine has been determined by crystallographic methods. The crystals are multifaced prisms, with orthorhombic space group P2(1)2(1)2(1) and unit cell dimensions of a = 17.98, b = 30.77 and c = 44.75A. The asymmetric unit consists of one duplex of hexanucleotide and 49 waters. The R-factor is 0.189 for 1495 reflections with F > or = sigma(F) to a resolution limit of 1.8A. The double helix has a Z-DNA type structure which appears to be intermediate in structure to the two previously characterised structure types for Z-DNA hexamers. The two m4C.G base-pairs adopt structures that are very similar to those of the equivalent base-pairs in the structure of the native sequence d(CGCGCG) except for the presence of the methyl groups which are trans to the N3 atoms of their parent nucleotides and protrude into the solvent region. The introduction of the modified base-pairs into the d(CGCGCG) duplex appears to have a minimal effect on the overall base-pair morphology of the Z-DNA duplex.

PubMed: 8284207
DOI: 10.1093/nar/21.24.5623

実験手法

X-RAY DIFFRACTION (1.8 Å)