11SO

EcMscM lacking the first periplasmic helical bundle in NaCl in a closed conformation

11SO の概要

• エントリーDOI: 10.2210/pdb11so/pdb
• 関連するPDBエントリー: 11SM
• EMDBエントリー: 76005 76007 76009
• 分子名称: Miniconductance mechanosensitive channel MscM (1 entity in total)
• 機能のキーワード: mechanosensitive, ion channel, mscs, membrane protein
• 由来する生物種: Escherichia coli K-12
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 706017.49

構造登録者

Hiotis, G.,Walz, T. (登録日: 2026-03-11, 公開日: 2026-08-05)

主引用文献

Hiotis, G.,Walz, T.
The bacterial mechanosensitive channel MscM gates through concerted changes in its transmembrane and cytoplasmic domains.
Nat Commun, 2026

PubMed Abstract: The mechanosensitive channel of small conductance (MscS) is the founding member of the family of MscS-like channels, which share a structurally conserved core but feature additional structural elements that define their specific channel characteristics. Here, we characterize the structure and function of the Escherichia coli mechanosensitive channel of mini conductance (MscM), which features eight additional transmembrane (TM) helices and a large periplasmic domain. Our cryo-EM structures reveal that channel gating involves conformational changes in all domains of MscM. In particular, a cytoplasmic extension of TM7 couples the conformation of the TM domain to that of the cytoplasmic domain, resulting in gating of its lateral fenestrations, where ions enter the channel. Thus, different from all other MscS-like channels studied to date, channel gating in MscM is mediated by its cytoplasmic domain and not the TM domain, which senses changes in membrane tension and operates the cytoplasmic gates.

PubMed: 42481478
DOI: 10.1038/s41467-026-75798-9

実験手法

ELECTRON MICROSCOPY (4.3 Å)