11FV

Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Pseudomonas aeruginosa (PA-S3.3)

これはPDB形式変換不可エントリーです。

11FV の概要

• エントリーDOI: 10.2210/pdb11fv/pdb
• EMDBエントリー: 75668
• 分子名称: 16S ribosomal RNA, Large ribosomal subunit protein bL25, Small ribosomal subunit protein uS3, ... (53 entities in total)
• 機能のキーワード: directed evolution, translation, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 52
• 化学式量合計: 2141493.88

構造登録者

Raskar, T. (登録日: 2026-02-21, 公開日: 2026-04-08)

主引用文献

Raskar, T.,Costello, A.,Badran, A.H.,Fraser, J.S.
Structural adaptations for enhanced translation kinetics in evolved ribosomes.
Biorxiv, 2026

PubMed Abstract: The ribosomal RNA sequence governs translation dynamics, yet understanding how changes beyond the conserved catalytic centers influence kinetics and protein yield remains limited. Using orthogonal ribosome phage-assisted continuous evolution (oRibo-PACE), we recently reported chimeric ribosomes derived from , , and endowed with elevated translation rates as compared to their starting counterparts. Here, we structurally characterize these kinetically enhanced ribosomes using cryo-electron microscopy and uncover a potential relationship between 16S rRNA stability and translation efficiency. Compared to their naive starting points, evolved ribosomes exhibit extensive RNA structural adaptation, often introduced by mismatches at key helical junctions, which leads to local RNA-protein rearrangements and destabilizes non-canonical base pairs. Compensatory mutations that restore base-pairing stability and eliminate flexibility reduced translational activity to wild-type levels. Across trajectories, increased translational output correlates with subtle, localized changes in the 16S rRNA sequence that introduce limited structural destabilization at specific elements. Taken together, our work provides new insights into rRNA structural malleability and establishes principles for engineering ribosomes with altered translation properties.

PubMed: 41846938
DOI: 10.64898/2026.03.05.706023

実験手法

ELECTRON MICROSCOPY (2.9 Å)