103D

THE UNUSUAL STRUCTURE OF THE HUMAN CENTROMERE (GGA)2 MOTIF: UNPAIRED GUANOSINE RESIDUES STACKED BETWEEN SHEARED G(DOT)A PAIRS

Summary for 103D

• Entry DOI: 10.2210/pdb103d/pdb
• Descriptor: DNA (5'-D(*GP*TP*GP*GP*AP*AP*TP*GP*GP*AP*AP*C)-3') (1 entity in total)
• Functional Keywords: dna, double helix, g-g stacking, g:a mismatch, human centromere repeat, ga-bracketed g-stack motif
• Total number of polymer chains: 2
• Total formula weight: 7502.93

Authors

Chou, S.-H.,Zhu, L.,Reid, B.R. (deposition date: 1994-12-16, release date: 1995-03-31, Last modification date: 2024-05-22)

Primary citation

Chou, S.H.,Zhu, L.,Reid, B.R.
The unusual structure of the human centromere (GGA)2 motif. Unpaired guanosine residues stacked between sheared G.A pairs.
J.Mol.Biol., 244:259-268, 1994

PubMed Abstract: The centromere of human chromosomes contains multiple repeats of the DNA sequence d(TGGAA)n. This sequence has the interesting property of pairing with itself to form stable duplexes. We have determined the solution structure of the unusual DNA duplex 5'-TGGAATGGAA:TGGAATGGAA-3' at atomic resolution. The duplex contains unpaired staggered guanosine residues, which co-stack by intercalation between sheared G.A and A.G base-pairs to form an interesting new structural motif, the GA-bracketed G-stack. The TGGAA repeat unit contains six "steps", four of which are not Watson-Crick base-pairs.

PubMed: 7966337
DOI: 10.1006/jmbi.1994.1727

Experimental method

SOLUTION NMR