5MGA
Structure of the Cpf1 endonuclease R-loop complex after DNA cleavage
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | CRISPR-associated endonuclease Cpf1 | polymer | 1323 | 154809.9 | 1 | UniProt (A0Q7Q2) Pfam (PF18501) Pfam (PF18516) Pfam (PF18510) In PDB | Francisella tularensis subsp. novicida U112 | FnCpf1 |
2 | B | RNA (40-MER) | polymer | 40 | 12808.6 | 1 | Francisella tularensis subsp. novicida U112 | ||
3 | C | DNA (26-MER) | polymer | 26 | 7937.1 | 1 | Francisella tularensis subsp. novicida U112 | ||
4 | D | DNA (5'-D(P*CP*GP*TP*TP*AP*GP*AP*GP*AP*AP*GP*T)-3') | polymer | 12 | 3726.5 | 1 | Francisella tularensis subsp. novicida U112 | ||
5 | B | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
6 | water | water | 18.0 | 130 | Chemie (HOH) |
Sequence modifications
A: 1 - 1300 (UniProt: A0Q7Q2)
PDB | External Database | Details |
---|---|---|
Asp 246 | Ile 246 | conflict |
Leu 467 | Lys 457 | conflict |
Ile 842 | Thr 842 | conflict |
Gly 1301 | - | expression tag |
Ser 1302 | - | expression tag |
Glu 1303 | - | expression tag |
Phe 1304 | - | expression tag |
Glu 1305 | - | expression tag |
Leu 1306 | - | expression tag |
Glu 1307 | - | expression tag |
Asn 1308 | - | expression tag |
Leu 1309 | - | expression tag |
Tyr 1310 | - | expression tag |
Phe 1311 | - | expression tag |
Gln 1312 | - | expression tag |
Gly 1313 | - | expression tag |
Glu 1314 | - | expression tag |
Leu 1315 | - | expression tag |
Arg 1316 | - | expression tag |
Arg 1317 | - | expression tag |
Gln 1318 | - | expression tag |
Ala 1319 | - | expression tag |
Ser 1320 | - | expression tag |
Ala 1321 | - | expression tag |
Leu 1322 | - | expression tag |
Glu 1323 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 179282.1 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 48.6 | |
All* | Total formula weight | 179330.7 |