3PSX

Crystal structure of the KT2 mutant of cytochrome P450 BM3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Bifunctional P-450/NADPH-P450 reductase	polymer	487	55503.2	2	UniProt (P14779) Pfam (PF00067) In PDB	Bacillus megaterium	Cytochrome P450(BM-3), Cytochrome P450BM-3, Cytochrome P450 102, NADPH-cytochrome P450 reductase
2	A, B	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	2	Chemie (HEM)
3		water	water		18.0	862	Chemie (HOH)

Sequence modifications

A, B: 0 - 481 (UniProt: P14779)

PDB	External Database	Details
Gly -5	-	expression tag
Pro -4	-	expression tag
Leu -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Thr 191	Ala 192	engineered mutation
His 239	Asn 240	engineered mutation
Val 259	Ile 260	engineered mutation
Thr 276	Ala 277	engineered mutation
Ile 353	Leu 354	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	111006.4
Non-Polymers*	Number of molecules	2
	Total formula weight	1233.0
All*	Total formula weight	112239.4

*Water molecules are not included.