2NOO

Crystal Structure of Mutant NikA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Nickel-binding periplasmic protein	polymer	502	56277.6	1	UniProt (P33590) Pfam (PF00496) In PDB	Escherichia coli K12	NikA
2	A	IODIDE ION	non-polymer		126.9	2	Chemie (IOD)
3	A	NICKEL (II) ION	non-polymer		58.7	1	Chemie (NI)
4		water	water		18.0	348	Chemie (HOH)

Sequence modifications

A: 1 - 502 (UniProt: P33590)

PDB	External Database	Details
Ala 22	Tyr 44	engineered mutation
Ala 97	Arg 119	engineered mutation
Ala 100	Trp 122	engineered mutation
Ala 137	Arg 159	engineered mutation
Tyi 300	Tyr 322	modified residue
Tyi 382	Tyr 404	modified residue
Ala 398	Trp 420	engineered mutation
Ala 402	Tyr 424	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	56277.6
Non-Polymers*	Number of molecules	3
	Total formula weight	312.5
All*	Total formula weight	56590.1

*Water molecules are not included.