3QX3

Human topoisomerase IIbeta in complex with DNA and etoposide

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
A	0005524	molecular_function	ATP binding
A	0006259	biological_process	DNA metabolic process
A	0006265	biological_process	DNA topological change
B	0003677	molecular_function	DNA binding
B	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
B	0005524	molecular_function	ATP binding
B	0006259	biological_process	DNA metabolic process
B	0006265	biological_process	DNA topological change

Functional Information from PDB Data

site_id	1
Number of Residues
Details

Chain

Residue

---

site_id	AC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE EVP A 1

Chain	Residue
A	GLY478
F	DG13
A	ASP479
A	ARG503
A	GLN778
A	MET782
C	DC8
D	DT9
D	DG10
F	DA12

---

site_id	AC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG A 1222

Chain	Residue
A	ASP557
A	ASP559

---

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG A 1223

Chain	Residue
A	ASN867
A	GLY868
A	GLU870

---

site_id	AC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG B 1

Chain	Residue
B	ASP557
B	ASP559

---

site_id	AC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MG B 1222

Chain	Residue
B	ASN867

---

site_id	AC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE EVP D 1

Chain	Residue
B	GLY478
B	ASP479
B	ARG503
B	MET782
D	DA12
D	DG13
E	DC8
F	DT9
F	DG10

---

Functional Information from PROSITE/UniProt

site_id	PS00177
Number of Residues	9
Details	TOPOISOMERASE_II DNA topoisomerase II signature. LTEGDSAKS

Chain	Residue	Details
A	LEU475-SER483

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: O-(5'-phospho-DNA)-tyrosine intermediate => ECO:0000255|PROSITE-ProRule:PRU01384, ECO:0000269|PubMed:21778401

Chain	Residue	Details
A	TYR821
B	TYR821

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00995

Chain	Residue	Details
A	GLU477
B	GLU477

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00995, ECO:0000269|PubMed:21778401

Chain	Residue	Details
A	ASP557
A	ASP559
B	ASP557
B	ASP559

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	SITE: Interaction with DNA

Chain	Residue	Details
A	LYS505
B	LYS678
B	LYS739
B	TRP947
A	ASN508
A	ARG677
A	LYS678
A	LYS739
A	TRP947
B	LYS505
B	ASN508
B	ARG677

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	SITE: Interaction with DNA => ECO:0000255|PROSITE-ProRule:PRU00995

Chain	Residue	Details
A	TYR773
B	TYR773

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	SITE: Transition state stabilizer

Chain	Residue	Details
A	ARG820
B	ARG820

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	SITE: Important for DNA bending; intercalates between base pairs of target DNA

Chain	Residue	Details
A	ILE872
B	ILE872

---

site_id	SWS_FT_FI8
Number of Residues	14
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
A	LYS595
B	LYS630
B	LYS641
B	LYS671
B	LYS707
B	LYS1087
A	LYS630
A	LYS641
A	LYS671
A	LYS707
A	LYS1087
B	LYS595

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25772364, ECO:0007744|PubMed:28112733

Chain	Residue	Details
A	LYS600
A	LYS638
B	LYS600
B	LYS638

---