3J95

Structure of ADP-bound N-ethylmaleimide sensitive factor determined by single particle cryoelectron microscopy

GO（遺伝子オントロジー）由来の情報

鎖名	GO（遺伝子オントロジー）id	名前空間	内容
A	0000149	molecular_function	SNARE binding
A	0000166	molecular_function	nucleotide binding
A	0001921	biological_process	positive regulation of receptor recycling
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005794	cellular_component	Golgi apparatus
A	0005795	cellular_component	Golgi stack
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006813	biological_process	potassium ion transport
A	0006886	biological_process	intracellular protein transport
A	0006891	biological_process	intra-Golgi vesicle-mediated transport
A	0015031	biological_process	protein transport
A	0016787	molecular_function	hydrolase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0017075	molecular_function	syntaxin-1 binding
A	0019901	molecular_function	protein kinase binding
A	0019905	molecular_function	syntaxin binding
A	0030165	molecular_function	PDZ domain binding
A	0030496	cellular_component	midbody
A	0035255	molecular_function	ionotropic glutamate receptor binding
A	0035494	biological_process	SNARE complex disassembly
A	0042802	molecular_function	identical protein binding
A	0043001	biological_process	Golgi to plasma membrane protein transport
A	0044877	molecular_function	protein-containing complex binding
A	0045732	biological_process	positive regulation of protein catabolic process
A	0046872	molecular_function	metal ion binding
A	0140545	molecular_function	ATP-dependent protein disaggregase activity
B	0000149	molecular_function	SNARE binding
B	0000166	molecular_function	nucleotide binding
B	0001921	biological_process	positive regulation of receptor recycling
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005794	cellular_component	Golgi apparatus
B	0005795	cellular_component	Golgi stack
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006813	biological_process	potassium ion transport
B	0006886	biological_process	intracellular protein transport
B	0006891	biological_process	intra-Golgi vesicle-mediated transport
B	0015031	biological_process	protein transport
B	0016787	molecular_function	hydrolase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0017075	molecular_function	syntaxin-1 binding
B	0019901	molecular_function	protein kinase binding
B	0019905	molecular_function	syntaxin binding
B	0030165	molecular_function	PDZ domain binding
B	0030496	cellular_component	midbody
B	0035255	molecular_function	ionotropic glutamate receptor binding
B	0035494	biological_process	SNARE complex disassembly
B	0042802	molecular_function	identical protein binding
B	0043001	biological_process	Golgi to plasma membrane protein transport
B	0044877	molecular_function	protein-containing complex binding
B	0045732	biological_process	positive regulation of protein catabolic process
B	0046872	molecular_function	metal ion binding
B	0140545	molecular_function	ATP-dependent protein disaggregase activity
C	0000149	molecular_function	SNARE binding
C	0000166	molecular_function	nucleotide binding
C	0001921	biological_process	positive regulation of receptor recycling
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005794	cellular_component	Golgi apparatus
C	0005795	cellular_component	Golgi stack
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006813	biological_process	potassium ion transport
C	0006886	biological_process	intracellular protein transport
C	0006891	biological_process	intra-Golgi vesicle-mediated transport
C	0015031	biological_process	protein transport
C	0016787	molecular_function	hydrolase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0017075	molecular_function	syntaxin-1 binding
C	0019901	molecular_function	protein kinase binding
C	0019905	molecular_function	syntaxin binding
C	0030165	molecular_function	PDZ domain binding
C	0030496	cellular_component	midbody
C	0035255	molecular_function	ionotropic glutamate receptor binding
C	0035494	biological_process	SNARE complex disassembly
C	0042802	molecular_function	identical protein binding
C	0043001	biological_process	Golgi to plasma membrane protein transport
C	0044877	molecular_function	protein-containing complex binding
C	0045732	biological_process	positive regulation of protein catabolic process
C	0046872	molecular_function	metal ion binding
C	0140545	molecular_function	ATP-dependent protein disaggregase activity
D	0000149	molecular_function	SNARE binding
D	0000166	molecular_function	nucleotide binding
D	0001921	biological_process	positive regulation of receptor recycling
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005794	cellular_component	Golgi apparatus
D	0005795	cellular_component	Golgi stack
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006813	biological_process	potassium ion transport
D	0006886	biological_process	intracellular protein transport
D	0006891	biological_process	intra-Golgi vesicle-mediated transport
D	0015031	biological_process	protein transport
D	0016787	molecular_function	hydrolase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0017075	molecular_function	syntaxin-1 binding
D	0019901	molecular_function	protein kinase binding
D	0019905	molecular_function	syntaxin binding
D	0030165	molecular_function	PDZ domain binding
D	0030496	cellular_component	midbody
D	0035255	molecular_function	ionotropic glutamate receptor binding
D	0035494	biological_process	SNARE complex disassembly
D	0042802	molecular_function	identical protein binding
D	0043001	biological_process	Golgi to plasma membrane protein transport
D	0044877	molecular_function	protein-containing complex binding
D	0045732	biological_process	positive regulation of protein catabolic process
D	0046872	molecular_function	metal ion binding
D	0140545	molecular_function	ATP-dependent protein disaggregase activity
E	0000149	molecular_function	SNARE binding
E	0000166	molecular_function	nucleotide binding
E	0001921	biological_process	positive regulation of receptor recycling
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005794	cellular_component	Golgi apparatus
E	0005795	cellular_component	Golgi stack
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0006813	biological_process	potassium ion transport
E	0006886	biological_process	intracellular protein transport
E	0006891	biological_process	intra-Golgi vesicle-mediated transport
E	0015031	biological_process	protein transport
E	0016787	molecular_function	hydrolase activity
E	0016887	molecular_function	ATP hydrolysis activity
E	0017075	molecular_function	syntaxin-1 binding
E	0019901	molecular_function	protein kinase binding
E	0019905	molecular_function	syntaxin binding
E	0030165	molecular_function	PDZ domain binding
E	0030496	cellular_component	midbody
E	0035255	molecular_function	ionotropic glutamate receptor binding
E	0035494	biological_process	SNARE complex disassembly
E	0042802	molecular_function	identical protein binding
E	0043001	biological_process	Golgi to plasma membrane protein transport
E	0044877	molecular_function	protein-containing complex binding
E	0045732	biological_process	positive regulation of protein catabolic process
E	0046872	molecular_function	metal ion binding
E	0140545	molecular_function	ATP-dependent protein disaggregase activity
F	0000149	molecular_function	SNARE binding
F	0000166	molecular_function	nucleotide binding
F	0001921	biological_process	positive regulation of receptor recycling
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005794	cellular_component	Golgi apparatus
F	0005795	cellular_component	Golgi stack
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0006813	biological_process	potassium ion transport
F	0006886	biological_process	intracellular protein transport
F	0006891	biological_process	intra-Golgi vesicle-mediated transport
F	0015031	biological_process	protein transport
F	0016787	molecular_function	hydrolase activity
F	0016887	molecular_function	ATP hydrolysis activity
F	0017075	molecular_function	syntaxin-1 binding
F	0019901	molecular_function	protein kinase binding
F	0019905	molecular_function	syntaxin binding
F	0030165	molecular_function	PDZ domain binding
F	0030496	cellular_component	midbody
F	0035255	molecular_function	ionotropic glutamate receptor binding
F	0035494	biological_process	SNARE complex disassembly
F	0042802	molecular_function	identical protein binding
F	0043001	biological_process	Golgi to plasma membrane protein transport
F	0044877	molecular_function	protein-containing complex binding
F	0045732	biological_process	positive regulation of protein catabolic process
F	0046872	molecular_function	metal ion binding
F	0140545	molecular_function	ATP-dependent protein disaggregase activity

PDBデータベースに由来する情報

site_id	AC1
残基数	17
詳細	BINDING SITE FOR RESIDUE ADP B 801

鎖名	残基
B	TYR502
B	SER547
B	GLY548
B	LYS549
B	THR550
B	ALA551
B	LEU552
B	ILE707
B	LYS708
B	ILE503
B	MET504
B	ASN505
B	GLY506
B	ILE507
B	ILE508
B	TRP510
B	HIS546

---

site_id	AC2
残基数	13
詳細	BINDING SITE FOR RESIDUE ADP C 801

鎖名	残基
C	ILE503
C	MET504
C	ASN505
C	GLY506
C	ILE507
C	ILE508
C	TRP510
C	VAL514
C	GLY548
C	LYS549
C	THR550
C	ALA551
C	LYS708

---

site_id	AC3
残基数	13
詳細	BINDING SITE FOR RESIDUE ADP D 801

鎖名	残基
D	TYR502
D	ILE503
D	MET504
D	GLY506
D	ILE507
D	ILE508
D	VAL514
D	HIS546
D	SER547
D	GLY548
D	LYS549
D	THR550
D	ALA551

---

site_id	AC4
残基数	14
詳細	BINDING SITE FOR RESIDUE ADP E 801

鎖名	残基
E	ASN505
E	GLY506
E	ILE507
E	TRP510
E	VAL514
E	HIS546
E	SER547
E	GLY548
E	LYS549
E	THR550
E	ALA551
E	LEU552
E	ILE707
E	LYS708

---

UniProtにおけるモチーフ・データベースPROSITEからの機能情報

site_id	PS00674
残基数	19
詳細	AAA AAA-protein family signature. IlVIgMTNrpdlIDeALl.R

鎖名	残基	詳細
A	ILE367-ARG385

---

SwissProt/UniProtに記載されている蛋白質分子機能情報

site_id	SWS_FT_FI1
残基数	12
詳細	BINDING: BINDING => ECO:0000269|PubMed:9731775, ECO:0000305|PubMed:9727495

鎖名	残基	詳細
A	ASN505
E	PRO545
F	ASN505
F	PRO545
A	PRO545
B	ASN505
B	PRO545
C	ASN505
C	PRO545
D	ASN505
D	PRO545
E	ASN505

---

site_id	SWS_FT_FI2
残基数	6
詳細	BINDING: BINDING => ECO:0000269|PubMed:9731775

鎖名	残基	詳細
A	THR550
B	THR550
C	THR550
D	THR550
E	THR550
F	THR550

---

site_id	SWS_FT_FI3
残基数	6
詳細	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P46460

鎖名	残基	詳細
A	LYS105
B	LYS105
C	LYS105
D	LYS105
E	LYS105
F	LYS105

---

site_id	SWS_FT_FI4
残基数	6
詳細	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P46459

鎖名	残基	詳細
A	SER207
B	SER207
C	SER207
D	SER207
E	SER207
F	SER207

---

site_id	SWS_FT_FI5
残基数	6
詳細	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P46460

鎖名	残基	詳細
A	TYR259
B	TYR259
C	TYR259
D	TYR259
E	TYR259
F	TYR259

---

site_id	SWS_FT_FI6
残基数	6
詳細	MOD_RES: Phosphoserine; by CDK16 => ECO:0000250|UniProtKB:P46460

鎖名	残基	詳細
A	SER569
B	SER569
C	SER569
D	SER569
E	SER569
F	SER569

---

CSAにおける酵素触媒機能の情報

site_id	MCSA1
残基数	6
詳細	M-CSA 642

鎖名	残基	詳細
A	LYS549	electrostatic stabiliser
A	THR550	electrostatic stabiliser
A	ASP603	electrostatic stabiliser
A	ASP604	proton acceptor
A	LYS631	electrostatic stabiliser
A	LYS708	electrostatic stabiliser

---

site_id	MCSA2
残基数	6
詳細	M-CSA 642

鎖名	残基	詳細
B	LYS549	electrostatic stabiliser
B	THR550	electrostatic stabiliser
B	ASP603	electrostatic stabiliser
B	ASP604	proton acceptor
B	LYS631	electrostatic stabiliser
B	LYS708	electrostatic stabiliser

---

site_id	MCSA3
残基数	6
詳細	M-CSA 642

鎖名	残基	詳細
C	LYS549	electrostatic stabiliser
C	THR550	electrostatic stabiliser
C	ASP603	electrostatic stabiliser
C	ASP604	proton acceptor
C	LYS631	electrostatic stabiliser
C	LYS708	electrostatic stabiliser

---

site_id	MCSA4
残基数	6
詳細	M-CSA 642

鎖名	残基	詳細
D	LYS549	electrostatic stabiliser
D	THR550	electrostatic stabiliser
D	ASP603	electrostatic stabiliser
D	ASP604	proton acceptor
D	LYS631	electrostatic stabiliser
D	LYS708	electrostatic stabiliser

---

site_id	MCSA5
残基数	6
詳細	M-CSA 642

鎖名	残基	詳細
E	LYS549	electrostatic stabiliser
E	THR550	electrostatic stabiliser
E	ASP603	electrostatic stabiliser
E	ASP604	proton acceptor
E	LYS631	electrostatic stabiliser
E	LYS708	electrostatic stabiliser

---

site_id	MCSA6
残基数	6
詳細	M-CSA 642

鎖名	残基	詳細
F	LYS549	electrostatic stabiliser
F	THR550	electrostatic stabiliser
F	ASP603	electrostatic stabiliser
F	ASP604	proton acceptor
F	LYS631	electrostatic stabiliser
F	LYS708	electrostatic stabiliser

---