Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1EJI

RECOMBINANT SERINE HYDROXYMETHYLTRANSFERASE (MOUSE)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
A	0004372	molecular_function	glycine hydroxymethyltransferase activity
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0006231	biological_process	dTMP biosynthetic process
A	0006544	biological_process	glycine metabolic process
A	0006545	biological_process	glycine biosynthetic process
A	0006563	biological_process	L-serine metabolic process
A	0006565	biological_process	L-serine catabolic process
A	0006730	biological_process	one-carbon metabolic process
A	0008270	molecular_function	zinc ion binding
A	0008732	molecular_function	L-allo-threonine aldolase activity
A	0009113	biological_process	purine nucleobase biosynthetic process
A	0016597	molecular_function	amino acid binding
A	0016740	molecular_function	transferase activity
A	0017148	biological_process	negative regulation of translation
A	0019264	biological_process	glycine biosynthetic process from serine
A	0030170	molecular_function	pyridoxal phosphate binding
A	0035999	biological_process	tetrahydrofolate interconversion
A	0036094	molecular_function	small molecule binding
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0046653	biological_process	tetrahydrofolate metabolic process
A	0046655	biological_process	folic acid metabolic process
A	0048027	molecular_function	mRNA 5'-UTR binding
A	0050897	molecular_function	cobalt ion binding
A	0051289	biological_process	protein homotetramerization
A	0070905	molecular_function	serine binding
A	1904482	biological_process	cellular response to tetrahydrofolate
A	1990830	biological_process	cellular response to leukemia inhibitory factor
B	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
B	0004372	molecular_function	glycine hydroxymethyltransferase activity
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005829	cellular_component	cytosol
B	0006231	biological_process	dTMP biosynthetic process
B	0006544	biological_process	glycine metabolic process
B	0006545	biological_process	glycine biosynthetic process
B	0006563	biological_process	L-serine metabolic process
B	0006565	biological_process	L-serine catabolic process
B	0006730	biological_process	one-carbon metabolic process
B	0008270	molecular_function	zinc ion binding
B	0008732	molecular_function	L-allo-threonine aldolase activity
B	0009113	biological_process	purine nucleobase biosynthetic process
B	0016597	molecular_function	amino acid binding
B	0016740	molecular_function	transferase activity
B	0017148	biological_process	negative regulation of translation
B	0019264	biological_process	glycine biosynthetic process from serine
B	0030170	molecular_function	pyridoxal phosphate binding
B	0035999	biological_process	tetrahydrofolate interconversion
B	0036094	molecular_function	small molecule binding
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0046653	biological_process	tetrahydrofolate metabolic process
B	0046655	biological_process	folic acid metabolic process
B	0048027	molecular_function	mRNA 5'-UTR binding
B	0050897	molecular_function	cobalt ion binding
B	0051289	biological_process	protein homotetramerization
B	0070905	molecular_function	serine binding
B	1904482	biological_process	cellular response to tetrahydrofolate
B	1990830	biological_process	cellular response to leukemia inhibitory factor
C	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
C	0004372	molecular_function	glycine hydroxymethyltransferase activity
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005829	cellular_component	cytosol
C	0006231	biological_process	dTMP biosynthetic process
C	0006544	biological_process	glycine metabolic process
C	0006545	biological_process	glycine biosynthetic process
C	0006563	biological_process	L-serine metabolic process
C	0006565	biological_process	L-serine catabolic process
C	0006730	biological_process	one-carbon metabolic process
C	0008270	molecular_function	zinc ion binding
C	0008732	molecular_function	L-allo-threonine aldolase activity
C	0009113	biological_process	purine nucleobase biosynthetic process
C	0016597	molecular_function	amino acid binding
C	0016740	molecular_function	transferase activity
C	0017148	biological_process	negative regulation of translation
C	0019264	biological_process	glycine biosynthetic process from serine
C	0030170	molecular_function	pyridoxal phosphate binding
C	0035999	biological_process	tetrahydrofolate interconversion
C	0036094	molecular_function	small molecule binding
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0046653	biological_process	tetrahydrofolate metabolic process
C	0046655	biological_process	folic acid metabolic process
C	0048027	molecular_function	mRNA 5'-UTR binding
C	0050897	molecular_function	cobalt ion binding
C	0051289	biological_process	protein homotetramerization
C	0070905	molecular_function	serine binding
C	1904482	biological_process	cellular response to tetrahydrofolate
C	1990830	biological_process	cellular response to leukemia inhibitory factor
D	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
D	0004372	molecular_function	glycine hydroxymethyltransferase activity
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005829	cellular_component	cytosol
D	0006231	biological_process	dTMP biosynthetic process
D	0006544	biological_process	glycine metabolic process
D	0006545	biological_process	glycine biosynthetic process
D	0006563	biological_process	L-serine metabolic process
D	0006565	biological_process	L-serine catabolic process
D	0006730	biological_process	one-carbon metabolic process
D	0008270	molecular_function	zinc ion binding
D	0008732	molecular_function	L-allo-threonine aldolase activity
D	0009113	biological_process	purine nucleobase biosynthetic process
D	0016597	molecular_function	amino acid binding
D	0016740	molecular_function	transferase activity
D	0017148	biological_process	negative regulation of translation
D	0019264	biological_process	glycine biosynthetic process from serine
D	0030170	molecular_function	pyridoxal phosphate binding
D	0035999	biological_process	tetrahydrofolate interconversion
D	0036094	molecular_function	small molecule binding
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0046653	biological_process	tetrahydrofolate metabolic process
D	0046655	biological_process	folic acid metabolic process
D	0048027	molecular_function	mRNA 5'-UTR binding
D	0050897	molecular_function	cobalt ion binding
D	0051289	biological_process	protein homotetramerization
D	0070905	molecular_function	serine binding
D	1904482	biological_process	cellular response to tetrahydrofolate
D	1990830	biological_process	cellular response to leukemia inhibitory factor

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	BINDING SITE FOR RESIDUE PLG A 500

Chain	Residue
A	SER53
A	SER119
A	GLY120
A	SER121
A	HIS148
A	THR202
A	SER203
A	ASP228
A	ALA230
A	HIS231
A	THR254
A	LYS257
A	ARG402
A	THF600
B	TYR73
B	TYR83
B	GLY302
B	GLY303

site_id	AC2
Number of Residues	16
Details	BINDING SITE FOR RESIDUE THF A 600

Chain	Residue
A	LEU143
A	GLY147
A	HIS148
A	LEU149
A	PHE153
A	LYS158
A	SER203
A	CYS204
A	ASN387
A	ALA395
A	LEU396
A	PLG500
B	GLU75
B	TYR82
B	TYR83
B	PRO298

site_id	AC3
Number of Residues	18
Details	BINDING SITE FOR RESIDUE PLG B 501

Chain	Residue
A	GLY302
A	GLY303
B	SER53
B	SER119
B	GLY120
B	SER121
B	HIS148
B	THR202
B	SER203
B	ASP228
B	ALA230
B	HIS231
B	THR254
B	LYS257
B	ARG402
B	THF601
A	TYR73
A	TYR83

site_id	AC4
Number of Residues	15
Details	BINDING SITE FOR RESIDUE THF B 601

Chain	Residue
A	GLU75
A	TYR82
A	HOH517
B	LEU143
B	GLY147
B	HIS148
B	LEU149
B	PHE153
B	LYS158
B	CYS204
B	ASN387
B	ALA395
B	ARG402
B	PLG501
B	HOH515

site_id	AC5
Number of Residues	18
Details	BINDING SITE FOR RESIDUE PLG C 502

Chain	Residue
C	SER53
C	SER119
C	GLY120
C	SER121
C	HIS148
C	THR202
C	SER203
C	ASP228
C	ALA230
C	HIS231
C	THR254
C	HIS256
C	LYS257
D	TYR73
D	GLU75
D	TYR83
D	GLY302
D	GLY303

site_id	AC6
Number of Residues	21
Details	BINDING SITE FOR RESIDUE PLG D 503

Chain	Residue
C	TYR73
C	GLU75
C	TYR83
C	GLY302
C	GLY303
C	HOH530
D	SER53
D	SER119
D	GLY120
D	SER121
D	ASN124
D	HIS148
D	HIS151
D	THR202
D	ASP228
D	ALA230
D	HIS231
D	THR254
D	HIS256
D	LYS257
D	ARG402

site_id	AC7
Number of Residues	12
Details	BINDING SITE FOR RESIDUE THF D 602

Chain	Residue
C	GLU75
C	TYR82
C	TYR83
D	LEU143
D	HIS148
D	LEU149
D	LYS158
D	ILE160
D	SER203
D	CYS204
D	ASN387
D	ARG402

site_id	I
Number of Residues	18
Details	ACTIVE SITE CONTAINING GLY-PLP COMPLEX AND THF INHIBITOR. SITE RESIDUES ARE FROM CHAINS A AND B.

Chain	Residue
A	SER53
A	HIS231
A	LYS257
A	ASN387
A	ARG402
B	TYR73
B	GLU75
B	TYR82
B	TYR83
B	GLY303
A	SER119
A	GLY120
A	SER121
A	GLY147
A	HIS148
A	LEU149
A	SER203
A	ASP228

site_id	II
Number of Residues	18
Details	ACTIVE SITE CONTAINING GLY-PLP COMPLEX AND THF INHIBITOR. SITE RESIDUES ARE FROM CHAINS B AND A.

Chain	Residue
B	SER53
B	SER119
B	GLY120
B	SER121
B	GLY147
B	HIS148
B	LEU149
B	SER203
B	ASP228
B	HIS231
B	LYS257
B	ASN387
B	ARG402
A	TYR73
A	GLU75
A	TYR82
A	TYR83
A	GLY303

site_id	III
Number of Residues	12
Details	ACTIVE SITE CONTAINING GLY-PLP COMPLEX ONLY. SITE RESIDUES ARE FROM CHAINS C AND D.

Chain	Residue
C	SER53
C	SER119
C	GLY120
C	SER121
C	HIS148
C	LEU149
C	ASP228
C	HIS231
C	LYS257
D	TYR73
D	TYR83
D	GLY303

site_id	IV
Number of Residues	15
Details	ACTIVE SITE CONTAINING GLY-PLP COMPLEX AND THF INHIBITOR. SITE RESIDUES ARE FROM CHAINS D AND C.

Chain	Residue
D	SER53
D	SER119
D	GLY120
D	SER121
D	HIS148
D	LEU149
D	SER203
D	ASP228
D	HIS231
D	LYS257
D	ARG402
C	TYR73
C	TYR82
C	TYR83
C	GLY303

Functional Information from PROSITE/UniProt

site_id	PS00096
Number of Residues	17
Details	SHMT Serine hydroxymethyltransferase pyridoxal-phosphate attachment site. HVvTTTTHKTLrGCRAG

Chain	Residue	Details
A	HIS249-GLY265

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	MOD_RES: N6-(pyridoxal phosphate)lysine => ECO:0000269\|PubMed:11063567

Chain	Residue	Details
A	LYS257
B	LYS257
C	LYS257
D	LYS257

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)