6QTS
Crystal structure of a mutant Arabidopsis WD40 domain in complex with a photoreceptor
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-06 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 48.343, 55.216, 103.017 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 43.760 - 1.110 |
| R因子 | 0.1283 |
| Rwork | 0.127 |
| R-free | 0.14790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5igo |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.059 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 43.760 | 1.150 |
| 分解能 [Å] (高) | 1.100 | 1.110 |
| 独立反射数 | 108152 | |
| <I/σ(I)> | 23.28 | |
| 完全性 [%] | 97.8 | |
| 冗長性 | 13.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 5 mg/mL of COP1 supplemented with 3 to 10 fold molar excess in peptide was mixed with two-fold (v/v) more mother liquor (1:2 ratio; protein:buffer) containing 2 M (NH4)2SO4 and 0.1 M HEPES pH 7.4 or 0.1M Tris pH 8.5. |
